Hygromycin resistance as an efficient selectable marker for wheat stable transformation

Autor:	Mónica M. Morata, Rubén H. Vallejos, Silva G. Altabe, Martín I. Reggiardo, Ricardo A. Ravizzini, Fernando E. Elias, Juan Pablo A. Ortiz, Marcelo A. Spitteler, Gerardo Domingo Lucio Cervigni
Rok vydání:	1995
Předmět:	Genetics Transgene fungi food and beverages Plant Science General Medicine Genetically modified crops Biology Molecular biology Transformation (genetics) Genetic marker Gene expression Agronomy and Crop Science Gene Selectable marker Southern blot
Zdroj:	Plant cell reports. 15(12)
ISSN:	0721-7714
Popis:	A highly efficient method for stable wheat transformation using hygromycin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder-driven microparticle accelerator. Transgenic wheat plants were determined by PCR amplification of transgene fragments and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relative to the number of bombarded calli) of 5.5% compared with 2.6% for the bar gene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3da1817bba65dad2a0b3925ad216d84e https://pubmed.ncbi.nlm.nih.gov/24178266 Zobrazit plný text záznamu Full text from SpringerLink