N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease
Autor: | Andrea Vukić Dugac, Toma Keser, Najda Selak, Olga Gornik, Gordan Lauc, Dario Dilber, Domagoj Kifer, Đivo Ljubičić, Tamara Pavić, Lada Rumora |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male Glycosylation Exacerbation Plasma glycoproteins lcsh:Medicine Disease N-glycosylation COPD biomarkers plasma glycoproteins immunoglobulin G General Biochemistry Genetics and Molecular Biology Immunoglobulin G COPD N-glycans HILIC-UPLC plasma proteins IgG 03 medical and health sciences Pulmonary Disease Chronic Obstructive 0302 clinical medicine N-linked glycosylation Polysaccharides Risk Factors Medicine Humans Risk factor Aged biology business.industry Research Smoking lcsh:R General Medicine Blood Proteins Middle Aged medicine.disease Blood proteins Glycome 3. Good health respiratory tract diseases 030104 developmental biology 030228 respiratory system Immunology biology.protein Female business Biomarkers |
Zdroj: | Journal of Translational Medicine, Vol 16, Iss 1, Pp 1-15 (2018) Journal of Translational Medicine |
Popis: | Background Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment. However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status. To overcome this limitation novel biomarkers for COPD have been investigated. Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls. Methods Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia. Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre. Results Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms). We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome. N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed. Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development. Conclusions This study showed that complexity of glycans associates with COPD, mirroring also the disease severity. Moreover, changes in N-glycome associate with exacerbation frequency and are affected by smoking. In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity. Electronic supplementary material The online version of this article (10.1186/s12967-018-1695-0) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
Externí odkaz: |