Plasmodium falciparum RIFIN is a novel ligand for inhibitory immune receptor LILRB2
Autor: | Kyoko Shida, Fumiji Saito, Sawako Itagaki, Kouyuki Hirayasu, Masako Kohyama, Wataru Nakai, Akihito Sakoguchi, Sumiko Matsuoka, Toshihiro Horii, Shiroh Iwanaga, Hisashi Arase, Tadahiro Suenaga |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Erythrocytes Plasmodium falciparum Protozoan Proteins Biophysics Human leukocyte antigen Immune receptor Ligands Biochemistry LILRB1 03 medical and health sciences 0302 clinical medicine Protein Domains LILRB2 parasitic diseases Animals Humans Malaria Falciparum Receptors Immunologic Receptor Molecular Biology Mice Inbred BALB C Membrane Glycoproteins biology LAIR1 Membrane Proteins Cell Biology Acquired immune system biology.organism_classification Cell biology HEK293 Cells 030104 developmental biology 030220 oncology & carcinogenesis Female Protein Binding |
Zdroj: | Biochemical and Biophysical Research Communications. 548:167-173 |
ISSN: | 0006-291X |
Popis: | Plasmodium falciparum causes the most severe form of malaria. Acquired immunity against P. falciparum provides insufficient protection even after repeated infections. Therefore, P. falciparum parasites might exploit inhibitory receptors for immune evasion. P. falciparum RIFINs are products of a multigene family consisting of 150–200 genes. Previously, we demonstrated that some RIFINs downregulate the immune response through the leukocyte immunoglobulin-like receptor (LILR) family inhibitory receptor, LILRB1, and leukocyte-associated immunoglobulin-like receptor 1, LAIR1. In this study, we further analyzed the expression of inhibitory receptor ligands on P. falciparum-infected erythrocytes and found that P. falciparum-infected erythrocytes expressed ligands for another LILR family inhibitory receptor, LILRB2, that recognizes HLA class I molecules as a host ligand. Furthermore, we identified that a specific RIFIN was a ligand for LILRB2 by using a newly developed RIFIN expression library. In addition, the domain 3 of LILRB2 was involved in RIFIN binding, whereas the domains 1 and 2 of LILRB2 were involved in the binding to HLA class I molecules. These results suggest that inhibitory receptor LILRB2 is also targeted by RIFIN for immune evasion of P. falciparum similar to LILRB1 and LAIR1. |
Databáze: | OpenAIRE |
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