Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis
| Autor: | Marie Mitsui, Yukihisa Shimada, Kazuo Soeno, Takahiro Ishii, Yusuke Kakei, Chiaki Yamazaki, Shin Takato, Masashi Suzuki, Yosuke Ishida, Ken-ichiro Hayashi, Ayako Nakamura |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Endogeny 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Biosynthesis Downregulation and upregulation Auxin Arabidopsis Gene expression heterocyclic compounds Molecular Biology chemistry.chemical_classification biology Organic Chemistry fungi food and beverages General Medicine biology.organism_classification 030104 developmental biology chemistry Signal transduction 010606 plant biology & botany Biotechnology |
| Zdroj: | NARO成果DBr C10201707260001_2473.pdf |
| DOI: | 10.6084/m9.figshare.4872851.v1 |
| Popis: | We previously reported that exogenous application of auxin to Arabidopsis seedlings resulted in downregulation of indole-3-acetic acid (IAA) biosynthesis genes in a feedback manner. In this study, we investigated the involvement of the SCFTIR1/AFB-mediated signaling pathway in feedback regulation of the indole-3-pyruvic acid-mediated auxin biosynthesis pathway in Arabidopsis. Application of PEO-IAA, an inhibitor of the IAA signal transduction pathway, to wild-type seedlings resulted in increased endogenous IAA levels in roots. Endogenous IAA levels in the auxin-signaling mutants axr2-1, axr3-3, and tir1-1afb1-1afb2-1afb3-1 also increased. Furthermore, YUCCA (YUC) gene expression was repressed in response to auxin treatment, and expression of YUC7 and YUC8 increased in response to PEO-IAA treatment. YUC genes were also induced in auxin-signaling mutants but repressed in TIR1-overexpression lines. These observations suggest that the endogenous IAA levels are regulated by auxin biosynthesis in a feedback manner, and the Aux/IAA and SCFTIR1/AFB-mediated auxin-signaling pathway regulates the expression of YUC genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|