Cell–cell adhesions in embryonic stem cells regulate the stability and transcriptional activity of β‐catenin

Autor: Sinjini Bhattacharyya, Ridim D. Mote, Jacob W. Freimer, Mahak Tiwari, Surya Bansi Singh, Sandhya Arumugam, Yadavalli V. Narayana, Raghav Rajan, Deepa Subramanyam
Rok vydání: 2022
Předmět:
Zdroj: FEBS Lett
ISSN: 1873-3468
0014-5793
DOI: 10.1002/1873-3468.14341
Popis: E-cadherin (CDH1) is involved in maintaining cell-cell adhesions in embryonic stem cells (ESCs). However, its function in the context of cell fate decisions is largely unknown. Using mouse ESCs (mESCs), we demonstrate that E-cadherin and β-catenin interact at the membrane and continue to do so upon internalization within the cell. Cdh1(−/−) mESCs failed to form tight colonies, with altered differentiation marker expression, and retention of pluripotency factors during differentiation. Interestingly, Cdh1(−/−) mESCs showed dramatically reduced β-catenin levels. Transcriptional profiling of Cdh1(−/−) mESCs displayed a significant alteration in the expression of a subset of β-catenin targets in a cell state- and GSK3β-dependent manner. Our findings hint at hitherto unknown roles played by E-cadherin in regulating the activity of β-catenin in ESCs.
Databáze: OpenAIRE
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