Parvalbumin basket cell myelination accumulates axonal mitochondria to internodes
| Autor: | Kole, Koen, Voesenek, Bas J B, Brinia, Maria E, Petersen, Naomi, Kole, Maarten H P, Sub Cell Biology, Celbiologie |
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| Přispěvatelé: | Sub Cell Biology, Celbiologie, Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Myelin Sheath/metabolism
Action Potentials/physiology Demyelinating Diseases/metabolism Multidisciplinary Action Potentials General Physics and Astronomy General Chemistry Axons/metabolism Interneurons/physiology Axons General Biochemistry Genetics and Molecular Biology Parvalbumins nervous system Interneurons Humans Parvalbumins/metabolism Myelin Sheath Demyelinating Diseases |
| Zdroj: | Nature Communications, 13(1). Nature Publishing Group |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-022-35350-x |
| Popis: | Parvalbumin-expressing (PV+) basket cells are fast-spiking inhibitory interneurons that exert critical control over local circuit activity and oscillations. PV+ axons are often myelinated, but the electrical and metabolic roles of interneuron myelination remain poorly understood. Here, we developed viral constructs allowing cell type-specific investigation of mitochondria with genetically encoded fluorescent probes. Single-cell reconstructions revealed that mitochondria selectively cluster to myelinated segments of PV+ basket cells, confirmed by analyses of a high-resolution electron microscopy dataset. In contrast to the increased mitochondrial densities in excitatory axons cuprizone-induced demyelination abolished mitochondrial clustering in PV+ axons. Furthermore, with genetic deletion of myelin basic protein the mitochondrial clustering was still observed at internodes wrapped by noncompacted myelin, indicating that compaction is dispensable. Finally, two-photon imaging of action potential-evoked calcium (Ca2+) responses showed that interneuron myelination attenuates both the cytosolic and mitochondrial Ca2+ transients. These findings suggest that oligodendrocyte ensheathment of PV+ axons assembles mitochondria to branch selectively fine-tune metabolic demands. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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