Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF-inhibitor to the rabbit tibia during distraction osteogenesis
| Autor: | Ebbe Stender Hansen, Martin Lind, Knud S. Christensen, Henrik Eckardt, Kristian G Bundgaard, Ivan Hvid |
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| Rok vydání: | 2003 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Bone Regeneration Bone density Angiogenesis medicine.medical_treatment Osteogenesis Distraction Neovascularization Physiologic Bone healing Bone Lengthening chemistry.chemical_compound Bone Density Internal medicine medicine Animals Orthopedics and Sports Medicine Therapeutic angiogenesis Antibodies Blocking Bone regeneration Tibia business.industry Microcirculation Elasticity Microspheres Recombinant Proteins Hindlimb Osteotomy Surgery Vascular endothelial growth factor Endocrinology chemistry Distraction osteogenesis Angiogenesis Inducing Agents Rabbits business |
| Zdroj: | Eckardt, E, Bundgaard, K G, Christensen, K S, Lind, M, Hansen, E S & Hvid, I 2003, ' Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF-inhibitor to the rabbit tibia during distraction osteogenesis ' Journal of Orthopaedic Research, vol. 21, no. 2, pp. 335-340 . Eckardt, H, Bundgaard, K G, Christensen, K S, Lind, M, Hansen, E S & Hvid, I 2003, ' Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF-inhibitor to the rabbit tibia during distraction osteogenesis ' Journal of Orthopaedic Research, vol. 21, no. 2, pp. 335-340 . |
| ISSN: | 1554-527X 0736-0266 |
| DOI: | 10.1016/s0736-0266(02)00159-6 |
| Popis: | Introduction: Therapeutic angiogenesis, a novel concept in tissue engineering, is neo-formation of blood vessels in a tissue upon delivery of an angiogenic growth factor to the tissue. We hypothesised that therapeutic angiogenesis could enhance bone formation and challenged the hypothesis in an experimental model of distraction osteogenesis. Methods: Rabbits, divided into three equal groups of 12, had their right tibia lengthened by distraction osteogenesis. A mini-osmotic pump delivered to the osteotomy gap either recombinant human vascular endothelial growth factor (VEGF), VEGF-inhibitor, or vehicle alone during the latency and distraction phase. After consolidation, we assessed bone blood flow by radioactive microsphere entrapment, measured torsional stiffness and bone mineral content, and did histomorphometry. Results: VEGF and VEGF-inhibitor treatment failed to influence bone blood flow, torsional stiffness, bone mineral content and histomorphometric indices of the bone regenerate. However, VEGF treatment increased the blood flow in bone of the distracted limb and VEGF-inhibitor treatment decreased bone blood flow. Conclusion: The regenerate was unresponsive to VEGF and VEGF-inhibitor treatment in contrast to the neighbouring bone, which implies different biological properties of the vasculature in native and regenerating bone. VEGF is not recommended for enhancement of bone formation in this setting. |
| Databáze: | OpenAIRE |
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