Microorganism adhesion using silicon dioxide: An experimental study
| Autor: | T. Selga, Roberts Lozins, Dzintars Ozoliņš |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Silicon dioxide Microorganism Microorganisms Microbiology Biochemistry Article Clinical research Absorbance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Spectrophotometry medicine lcsh:Social sciences (General) Bacterial types lcsh:Science (General) Infectious disease Multidisciplinary Chromatography Bacteria medicine.diagnostic_test biology Chemistry Near-infrared spectroscopy Laboratory medicine biology.organism_classification Yeast 030104 developmental biology lcsh:H1-99 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 4, Pp e03678-(2020) Heliyon |
| ISSN: | 2405-8440 |
| DOI: | 10.1016/j.heliyon.2020.e03678 |
| Popis: | In this study, spectrophotometry was used to measure changes in the absorbance properties of yeast, Gram-positive, and Gram-negative bacteria after their attachment to silicon dioxide microparticles (silica). The goal of this study was to determine whether spectrophotometry is an effective method to distinguish these microorganisms from one another and determine whether they have an affinity for silicon dioxide. The experiments were performed by examining the light absorption properties of yeast, Gram-positive and Gram-negative bacteria in a spectrophotometer, both with and without silicon dioxide microparticles. The experiments produced a number of promising results. First, the spectrophotometer graphs of yeast were noticeably different from those of both Gram-positive and Gram-negative bacteria. Second, the absorption of light in both Gram-positive and Gram-negative bacteria occurred at near infrared range (700–1500 nm) and, unlike yeast, the wavelengths increased when silicon dioxide microparticles were added to the suspension. When silicon dioxide microparticles were added to yeast, the absorption of light decreased during the entire wavelength interval of the spectrophotometer measurement. These results indicate that bacteria have an affinity for silicon dioxide, and that spectrophotometry may be used to distinguish yeast from bacteria and, possibly, different bacterial types from one another. Microbiology; Biochemistry; Infectious disease; Laboratory medicine; Clinical research; Silicon dioxide; Spectrophotometry; Microorganisms; Yeast; Bacteria |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|