Toxoplasma gondii secretes a calcium-independent phospholipase A 2
| Autor: | Sophie Cassaing, Josette Fauvel, Marie-Hélène Bessières, Sandrine Guy, Hugues Chap, J. P. Seguela |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Phospholipid
chemistry.chemical_element Phospholipase Calcium Polymerase Chain Reaction Phospholipases A Microbiology Calcium Chloride Mice chemistry.chemical_compound Phospholipase A2 parasitic diseases Animals Fluorometry DNA Primers Electrophoresis Agar Gel chemistry.chemical_classification Phosphatidylglycerol Mice Inbred BALB C Phospholipase A Deoxyribonuclease BamHI biology Toxoplasma gondii DNA Protozoan Hydrogen-Ion Concentration biology.organism_classification Mice Inbred C57BL Toxoplasmosis Animal Infectious Diseases Enzyme chemistry Macrophages Peritoneal biology.protein Female lipids (amino acids peptides and proteins) Parasitology Chromatography Thin Layer Toxoplasma |
| Zdroj: | International Journal for Parasitology. 30:1137-1142 |
| ISSN: | 0020-7519 |
| Popis: | Phospholipases A(2) (PLA(2)) play an important role in Toxoplasma gondii host cell penetration. They are also key enzymes in the host cell response to the parasite invasion. PLA(2) hydrolyse cellular phospholipids, releasing multiple inflammatory lipidic mediators. We have investigated the biochemical characterisation of T. gondii PLA(2) activity in a mouse-cultured tachyzoite homogenate and in the peritoneal exudate from infected mice, using the hydrolysis of a fluorescent phosphatidylglycerol labelled at the sn-2 position. Spectrofluorimetry and thin-layer chromatography showed a PLA(2) activity (about 0.5-2 nmol/min per mg), calcium-independent, secreted into infected mice peritoneal exudate, with a broad pH activity ranging between 6.5 and 9.5 and resistant to a great number of potential PLA(2) inhibitors except dithio-nitrobenzoic acid (1 mM). An associated phospholipase A(1) activity was also displayed. These results suggest that Toxoplasma gondii displays specific phospholipases different from host enzymes and probably involved at critical steps of infectious cycle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect