Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide
Autor: | Laura Villamizar, Gloria Barrera, Mark R. H. Hurst, Travis R. Glare |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Larva
biology Strain (chemistry) microsclerotia grass grubs QH301-705.5 fungi food and beverages diamond back moth biology.organism_classification Porina Microbiology QR1-502 metarhizium Biopesticide Infectious Diseases entomopathogenic fungus porina parasitic diseases Entomopathogenic fungus Botany Metarhizium Biology (General) Research Article |
Zdroj: | Mycology, Vol 12, Iss 4, Pp 261-278 (2021) Mycology article-version (VoR) Version of Record |
ISSN: | 2150-1211 2150-1203 |
Popis: | The fungal species Metarhizium novozealandicum, that occurs only in New Zealand and Australia has been poorly studied. In this work, a new strain of M. novozealandicum isolated from a larva of Wiseana sp. is described based on morphology, genomic multilocus (ITS, EF-1α and β-tubulin) phylogeny, growth in different culture media and insecticidal activity. The isolate AgR-F177 was clustered in the same clade with M. novozealandicum. AgR-F177 colonies developed faster on Sabouraud Dextrose Agar (SDA) than on Potato Dextrose Agar (PDA) when incubated at 25°C, with no growth observed at 30°C on either media. Conidia yield on an oat-based medium in semisolid fermentation was 7.41 x 108conidia/g of substrate and a higher yield of 1.68 x 109conidia/g of substrate was obtained using solid fermentation on cooked rice. AgR-F177 formed microsclerotia (MS) in liquid fermentation after 7 days reaching the maximum yield of 3.3 × 103 MS/mL after 10 days. AgR-F177 caused mortality in Wiseana copularis, Costelytra giveni and Plutella xylostella larvae with efficacies up to 100%, 69.2%, and 45.7%, respectively. The ease of production of AgR-F177 with different fermentation systems and its pathogenicity against different insect pests reveal its potential as a new biopesticide. |
Databáze: | OpenAIRE |
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