Effects of Shaoyao-Gancao Decoction on Infarcted Cerebral Cortical Neurons: Suppression of the Inflammatory Response following Cerebral Ischemia-Reperfusion in a Rat Model
| Autor: | Xinling Jia, Jingye Wang, Jian Yang, Zhongju Xu, Qing Li, Ying Zhang, Guofeng Yan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A Interleukin-1beta lcsh:Medicine Brain Ischemia Brain ischemia Rats Sprague-Dawley 0302 clinical medicine Chemokine CCL5 Chemokine CCL2 Neurons Microglia Glial fibrillary acidic protein biology Cerebral infarction Infarction Middle Cerebral Artery General Medicine Cerebral Infarction Interleukin-10 medicine.anatomical_structure Anesthesia Nissl body symbols medicine.symptom Research Article medicine.medical_specialty Article Subject Ischemia Inflammation General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine Transforming Growth Factor beta1 03 medical and health sciences symbols.namesake Internal medicine Glial Fibrillary Acidic Protein medicine Animals General Immunology and Microbiology business.industry Tumor Necrosis Factor-alpha lcsh:R medicine.disease Rats 030104 developmental biology Endocrinology Astrocytes Reperfusion biology.protein business 030217 neurology & neurosurgery Drugs Chinese Herbal |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2016 (2016) |
| ISSN: | 2314-6141 |
| Popis: | The mechanisms by which Shaoyao-Gancao decoction (SGD) inhibits the production of inflammatory cytokines in serum and brain tissue after cerebral ischemia-reperfusion (CI-RP) in rats were investigated. A right middle cerebral artery occlusion was used to induce CI-RP after which the rats were divided into model (n=39), SGD (n=28), clopidogrel (n=25) and sham operated (n=34) groups. The Bederson scale was used to evaluate changes in behavioral indices. The levels of IL-1β, TNF-α, MCP-1, IL-10, RANTES, VEGF, and TGF-β1 in the serum and infarcted brain tissues were measured. Nissl body and immunohistochemical staining methods were used to detect biochemical changes in neurons, microglial cells, and astrocytes. Serum levels of VEGF, TNF-α, MCP-1, IL-1β, and IL-10 increased significantly 24 h after CI-RP. In brain tissue, levels of TNF-αand IL-1βsignificantly increased 24 h after CI-RP, whereas levels of TGF-β1 and MCP-1 were significantly higher 96 h after CI-RP (P<0.05). SGD or clopidogrel after CI-RP reduced TNF-αand IL-1βlevels in brain tissue and serum levels of MCP-1, IL-1β, and IL-10. SGD increased the number of NeuN-positive cells in infarcted brain tissue and reduced the number of IBA1-positive and GFAP-positive cells. The efficacy of SGD was significantly higher than that of clopidogrel. |
| Databáze: | OpenAIRE |
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