Imaging of zinc oxide nanoparticle penetration in human skin in vitro and in vivo
| Autor: | Washington Y. Sanchez, Xin Zhao, Andrei V. Zvyagin, Michael S. Roberts, Audrey Gierden, Justin A. Ross |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Materials science
Scanning electron microscope Administration Topical Skin Absorption Biomedical Engineering chemistry.chemical_element Nanoparticle Nanotechnology Human skin Zinc Biomaterials In vivo Microscopy Stratum corneum medicine Humans Tissue Distribution Skin integumentary system Penetration (firestop) Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Microscopy Electron medicine.anatomical_structure Microscopy Fluorescence chemistry Biophysics Nanoparticles Zinc Oxide |
| Zdroj: | Journal of Biomedical Optics. 13:064031 |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.3041492 |
| Popis: | Zinc oxide ZnO-nano and titanium dioxide nanoparticles 20 to 30 nm are widely used in several topical skin care products, such as sunscreens. However, relatively few studies have addressed the subdermal absorption of these nanoparticles in vivo. We report on investigation of the distribution of topically applied ZnO in excised and in vivo human skin, using multiphoton microscopy MPM imag- ing with a combination of scanning electron microscopy SEM and an energy-dispersive x-ray EDX technique to determine the level of penetration of nanoparticles into the sub-dermal layers of the skin. The good visualization of ZnO in skin achieved appeared to result from two factors. First, the ZnO principal photoluminescence at 385 nm is in the "quiet" spectral band of skin autofluorescence domi- nated by the endogenous skin fluorophores, i.e., NADPH and FAD. Second, the two-photon action cross section of ZnO-nano ZnO TPEF 0.26 GM; diameter, 18 nm is high: 500-fold of that inferred from its bulk third-order nonlinear susceptibility Im ZnO 3 , and is favorably compared to that of NADPH and FAD. The overall out- come from MPM, SEM, and EDX studies was that, in humans in vivo, ZnO nanoparticles stayed in the stratum corneum SC and accumu- lated into skin folds and/or hair follicle roots of human skin. Given the lack of penetration of these nanoparticles past the SC and that the outermost layers of SC have a good turnover rate, these data suggest that the form of ZnO-nano studied here is unlikely to result in safety concerns. © 2008 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.3041492 |
| Databáze: | OpenAIRE |
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