Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis
| Autor: | Emeline Tanguy, Sylvette Chasserot-Golaz, Laetitia Fouillen, Tamou Thahouly, Dimitri Heintz, Marie-France Bader, Nicolas Vitale, Nawal Kassas, Nancy J. Grant |
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| Přispěvatelé: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie moléculaire des plantes (IBMP) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Recombinant Fusion Proteins Phagocytosis Green Fluorescent Proteins Phospholipid Phosphatidic Acids Biosensing Techniques Biology Second Messenger Systems Biochemistry Green fluorescent protein 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Humans Molecular Biology Phospholipase D Macrophages Cell Membrane Cell Biology Phosphatidic acid Lipid signaling Lipids 030104 developmental biology chemistry Second messenger system Biophysics [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (10), ⟨10.1074/jbc.M116.742346⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.m116.742346 |
| Popis: | Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterized in vitro, and their preferences for different PA species in particular lipidic environments were compared. In addition, the localization of PA in macrophages during frustrated phagocytosis was examined using these PA sensors and was combined with a lipidomic analysis of PA in intracellular compartments. The results indicate that the PA sensors display some preferences for specific PA species, depending on the lipid environment, and the localization study in macrophages revealed the complexity of intracellular PA dynamics. |
| Databáze: | OpenAIRE |
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