R432 is a key residue for the multiple functions of Ndt80p in Candida albicans
| Autor: | Ting Yin Hsiao, Chao Agnes Hsiung, Chih Yu Chen, Hsiu-Jung Lo, Chih Wei Wang, Shiang Ning Leaw, Chiung Tong Chen, Szu Hsuan Huang, Chwan Deng Hsiao, Te Pin Chang, Kuo Yun Tseng, Jen Chung Fan, Chia Geun Chen, I Chin Wang, Yun-Liang Yang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Hyphal growth Hyphae Virulence Germ tube Nitric Oxide Microbiology Mice Cellular and Molecular Neuroscience Transcription (biology) Candida albicans Animals Protein–DNA interaction Molecular Biology Gene Pharmacology Mice Inbred BALB C Fungal protein biology DNA Cell Biology biology.organism_classification Cell biology DNA-Binding Proteins Molecular Medicine Transcription Factors |
| Zdroj: | Cellular and Molecular Life Sciences. 69:1011-1023 |
| ISSN: | 1420-9071 1420-682X |
| DOI: | 10.1007/s00018-011-0849-5 |
| Popis: | Ndt80p is an important transcription modulator to various stress-response genes in Candida albicans, the most common human fungal pathogen in systemic infections. We found that Ndt80p directly regulated its target genes, such as YHB1, via the mid-sporulation element (MSE). Furthermore, the ndt80 R432A allele, with a reduced capability to bind MSE, failed to complement the defects caused by null mutations of NDT80. Thus, the R432 residue in the Ndt80p DNA-binding domain is involved in all tested functions, including cell separation, drug resistance, nitric oxide inactivation, germ tube formation, hyphal growth, and virulence. Hence, the importance of the R432 residue suggests a novel approach for designing new antifungal drugs by blocking the interaction between Ndt80p and its targets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink