Influence of sialic acid removal on MUC1 antigenic reactivity in head and neck carcinoma
| Autor: | Adrián Pereyra, María Virginia Croce, Amada Segal-Eiras, Martín Enrique Rabassa |
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| Předmět: |
Male
Cancer Research medicine.drug_class Blotting Western Monoclonal antibody Epitope Pathology and Forensic Medicine chemistry.chemical_compound Epitopes Antigen medicine Humans Antigens Tumor-Associated Carbohydrate Aged biology Mucin-1 Antibodies Monoclonal General Medicine Middle Aged medicine.disease Molecular biology Head and neck squamous-cell carcinoma N-Acetylneuraminic Acid Sialic acid Oncology Biochemistry chemistry Head and Neck Neoplasms biology.protein Carcinoma Squamous Cell Electrophoresis Polyacrylamide Gel Female Binding Sites Antibody Antibody Neuraminidase Hapten |
| Zdroj: | Scopus-Elsevier |
| Popis: | To investigate the influence of sialic acid removal on MUC1 peptidic and carbohydrate epitope reactivity in head and neck squamous cell carcinoma (HNSCC), tumor samples belonging to 24 HNSCC patients were studied by standard immunohistochemistry (IHC) with and without desialylation with 0.1 U/ml neuraminidase. From each tumor sample, subcellular fractions were obtained and analyzed by SDS-PAGE and Western blotting (WB). Three monoclonal antibodies (MAbs) were used: C595 MAb directed to MUC1 protein core, an antiTn hapten MAb, and an anti-sTn hapten MAb; a comparative analysis between desialylated and sialylated samples was performed. By IHC without neuraminidase treatment, 19 of 24 samples reacted with anti-MUC1 peptidic epitope, while Tn hapten was not detected and sTn was found in 1 of 24 cases. Desialylation increased either the number of reacting cells or the intensity of the reaction with C595 and anti-Tn MAbs, and some negative samples became positive. On the other hand, sTn expression decreased with desialylation. By WB, several bands from >200 to 25 kDa were found; desialylation increased high-molecular-weight bands, diminishing the detection of low-molecular-weight ones. The use of desialylation is a suitable treatment that contributes to the exposure of MUC1-associated epitopes, which may be related to the spreading of HNSCC. (Pathology Oncology Research Vol 11, No 2, 74–81) |
| Databáze: | OpenAIRE |
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