Vitamin D promotes autophagy in AML cells by inhibiting miR-17-5p-induced Beclin-1 overexpression
| Autor: | Yong Yuan, Jinye Xie, Jing Liu, Kang Chen, Juan Wang, Qian Dong, Weijia Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Clinical Biochemistry Regulator Apoptosis 03 medical and health sciences 0302 clinical medicine Cell Line Tumor hemic and lymphatic diseases microRNA Autophagy Biomarkers Tumor Vitamin D and neurology Humans Vitamin D Molecular Biology Cell Proliferation Chemistry Cell growth Myeloid leukemia Vitamins Cell Biology General Medicine BECN1 Middle Aged Survival Rate Leukemia Myeloid Acute MicroRNAs 030104 developmental biology ROC Curve Case-Control Studies 030220 oncology & carcinogenesis Cancer research Beclin-1 Female |
| Zdroj: | Molecular and Cellular Biochemistry. 476:3951-3962 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | MicroRNA (miR)-17-5p has been investigated in many diseases as a regulator of disease progression and is highly expressed in acute myeloid leukemia (AML). However, potential mechanisms underlying the function of miR-17-5p in AML need more elucidation. MiR-17-5p expression was augmented, while 25(OH)D3 and Beclin-1 levels were decreased in AML patients with the highest risk for disease progression. MiR-17-5p, 25(OH)D3 and Beclin-1 were determined to be clinically important in AML based on ROC curve analysis. Higher miR-17-5p expression as well as lower 25(OH)D3 and Beclin-1 expression were relevant with poor prognosis in AML. In addition, miR-17-5p was negatively correlated with and bound to BECN1. Vitamin D was found to diminish cell proliferation and enhance autophagy. Finally, through rescue assays, miR-17-5p facilitated the ability of cell proliferation, inhibited autophagy and apoptosis by modulating Beclin-1 in HL-60 cells following the treatment of 4 μM vitamin D. Vitamin D promoted autophagy in AML cells by modulating miR-17-5p and Beclin-1. |
| Databáze: | OpenAIRE |
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