RNA Interference of the Muscle Actin Gene in Bed Bugs: Exploring Injection Versus Topical Application for dsRNA Delivery
| Autor: | Sanjay Basnet, Shripat T. Kamble |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Bedbugs media_common.quotation_subject Insect 03 medical and health sciences Bed bug RNA interference parasitic diseases Animals gene knockdown topical application Gene Muscle actin media_common RNA Double-Stranded Gene knockdown biology fungi General Medicine biology.organism_classification Actins Cell biology RNA silencing 030104 developmental biology bed bug injection Insect Science RNAi Female RNA Interference Functional genomics Research Article |
| Zdroj: | Journal of Insect Science |
| ISSN: | 1536-2442 |
| Popis: | Bed bugs are one the most troublesome household pests that feed primarily on human blood. RNA interference (RNAi) is currently being pursued as a potential tool for insect population management and has shown efficacy against some phytophagous insects. We evaluated the different techniques to deliver dsRNA specific to bed bug muscle actin (dsactin) into bed bugs. Initially, stability of dsRNA in human blood was studied to evaluate the feasibility of feeding method. Adult bed bugs were injected with dsRNA between last thoracic segment and first abdominal segment on the ventral side, with a dose of 0.2 µg dsactin per insect. In addition to injection, dsactin was mixed in acetone and treated topically in the abdomens of fifth stage nymphs. We found the quick degradation of dsRNA in blood. Injection of dsactin caused significant depletion of actin transcripts and substantial reduction in oviposition and lethality in female adults. Topically treated dsRNA in fifth stage nymphs had no effect on actin mRNA expression and survival. Our results demonstrated that injection is a reliable method of dsRNA delivery into bed bugs while topical treatment was not successful. This research provides an understanding on effective delivery methods of dsRNA into bed bugs for functional genomics research and feasibility of the RNAi based molecules for pest management purposes. |
| Databáze: | OpenAIRE |
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