High expression of Mcl-1 in ALK positive and negative anaplastic large cell lymphoma
| Autor: | Tjasso Blokzijl, Sibrand Poppema, A.M. van den Berg, Joost Kluiver, M Boot, Megan S. Lim, S-C Peh, WA Kamps, Lydia Visser, Arjan Diepstra, Renata Rust, Geertruida Harms |
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| Přispěvatelé: | University of Groningen, Stem Cell Aging Leukemia and Lymphoma (SALL), Translational Immunology Groningen (TRIGR) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
CD4-Positive T-Lymphocytes
bcl-X Protein BCL-2 FAMILY PROTEINS Biology UP-REGULATION MYELOMA CELLS Pathology and Forensic Medicine DIFFERENTIAL EXPRESSION STAT3 Cell Line Tumor hemic and lymphatic diseases Gene expression medicine Humans Anaplastic Lymphoma Kinase HEMATOPOIETIC-CELLS RNA Messenger RNA Neoplasm Serial analysis of gene expression Anaplastic large-cell lymphoma GENE-EXPRESSION Reverse Transcriptase Polymerase Chain Reaction Large-cell lymphoma Receptor Protein-Tyrosine Kinases CDNA ARRAYS Original Articles General Medicine Protein-Tyrosine Kinases KI-1 LYMPHOMA medicine.disease Immunohistochemistry Genes bcl-2 Neoplasm Proteins APOPTOSIS Gene Expression Regulation Neoplastic Reverse transcription polymerase chain reaction Proto-Oncogene Proteins c-bcl-2 Cell culture Cancer research Myeloid Cell Leukemia Sequence 1 Protein Lymphoma Large B-Cell Diffuse SAGE Library |
| Zdroj: | Journal of Clinical Pathology, 58(5), 520-524. BMJ PUBLISHING GROUP |
| ISSN: | 0021-9746 |
| Popis: | Aim: To gain more insight into the genes involved in the aetiology and pathogenesis of anaplastic large cell lymphoma (ALCL).Methods: Serial analysis of gene expression ( SAGE) was undertaken on the CD4+ALK+ ( anaplastic lymphoma kinase positive) ALCL derived cell line Karpas299 and as comparison on CD4+ T cells. Quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were performed on five ALCL derived cell lines and 32 tissue samples to confirm the SAGE data.Results: High expression of Mcl-1 was seen in the Karpas299 cell line, whereas the two other antiapoptotic Bcl-2 family members, Bcl-2 and Bcl-X-L, were not detected in the SAGE library. Quantitative RT-PCR confirmed the high expression of Mcl-1 mRNA and low expression of Bcl-2 and Bcl-X-L in Karpas299 and in four other ALCL cell lines. To expand on these initial observations, primary tissue samples were analysed for Mcl-1, Bcl-X-L, and Bcl-2 by immunohistochemistry. All 23 ALK+ and nine ALK2 ALCL cases were positive for Mcl-1. Bcl-2 and Bcl-X-L were expressed infrequently in ALK+ ALCL cases, but were present in a higher proportion of ALK2 ALCL cases.Conclusion: The consistent high expression of Mcl-1 in ALK+ and ALK2 ALCL suggests that Mcl-1 is the main antiapoptotic protein in this disease. The high frequency of Mcl-1, Bcl-2, and Bcl-X-L positive ALCL cases in the ALK2 group compared with the ALK+ group indicates that ALK induced STAT3 activation is not the main regulatory pathway in ALCL. |
| Databáze: | OpenAIRE |
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