Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment
| Autor: | Liliana Grenho, Pedro S. Gomes, Catarina C. Coelho, Paulo A. Quadros, Maria Helena Fernandes |
|---|---|
| Přispěvatelé: | Instituto de Investigação e Inovação em Saúde |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fibroblasts / drug effects Durapatite / toxicity media_common.quotation_subject In vitro cytotoxicity lcsh:Medicine Biocompatible Materials Oral Health Cosmetics Nanoparticles / toxicity Pharmacology medicine.disease_cause Article 03 medical and health sciences Ingredient 0302 clinical medicine medicine Humans lcsh:Science Nanotoxicology Cytotoxicity Hydroxyapatite nanoparticles Normal behaviour media_common Multidisciplinary Chemistry lcsh:R Fibroblasts stomatognathic diseases Durapatite 030104 developmental biology Nano hydroxyapatite Consumer Product Safety Nanoparticles lcsh:Q Cosmetics / toxicity Biocompatible Materials / toxicity Irritation 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Nano-hydroxyapatite has been used as an oral care ingredient, being incorporated in several products for the treatment of dental hypersensitivity and enamel remineralisation. Despite its promising results, regulatory and safety concerns have been discussed and questioned by the European Scientific Committee on Consumer Safety (SCCS) regarding the usage of hydroxyapatite nanoparticles in oral care products. In this work, a commercially available nano-hydroxyapatite was characterized and its cytocompatibility towards human gingival fibroblasts was evaluated, as well as its irritation potential using the in vitro HET-CAM assay. All the conditions chosen in this study tried to simulate the tooth brushing procedure and the hydroxyapatite nanoparticles levels normally incorporated in oral care products. The commercial hydroxyapatite nanoparticles used in this study exhibited a rod-like morphology and the expected chemical and phase composition. The set of in vitro cytotoxicity parameters accessed showed that these nanoparticles are highly cytocompatible towards human gingival fibroblasts. Additionally, these nanoparticles did not possess any irritation potential on HET-CAM assay. This study clarifies the issues raised by SCCS and it concludes that this specific nano-hydroxyapatite is cytocompatible, as these nanoparticles did not alter the normal behaviour of the cells. Therefore, they are safe to be used in oral care products. The authors acknowledge the support of the Biointerfaces and Nanotechnology i3S Scientific Platform, as well as Luís Teixeira and Marta Ferro from University of Aveiro for the characterization of the HA-NP with TEM. Financial support from the European Union (FEDER funds POCI/01/0145/FEDER/007265) and National Funds (FCT/MEC, Fundação para a Ciência e Tecnologia and Ministério da Educação e Ciência) under the Partnership Agreement PT2020 UID/QUI/50006/2019 is acknowledged. |
| Databáze: | OpenAIRE |
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