Properties of natural and hybrid murine alpha interferons
| Autor: | Ellen C. Zwarthoff, Margreet van Heuvel, I. Jaap Bosveld, Jan Trapman, Arno T. A. Mooren |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
COS cells DNA Recombinant Alpha interferon Biology Virology Molecular biology Vesicular stomatitis Indiana virus Amino acid Cell Line Mice Secretory protein chemistry Interferon Cell culture Cricetinae Gene expression Interferon Type I medicine Animals Polyacrylamide gel electrophoresis medicine.drug Plasmids |
| Zdroj: | The Journal of general virology. 67 |
| ISSN: | 0022-1317 |
| Popis: | Four natural murine interferon-alpha genes (MuIFN-alpha 1, -alpha 2, -alpha 4 and -alpha 6) and four hybrid genes (alpha 1 alpha 4, alpha 2 alpha 4, alpha 4 alpha 1 and alpha 4 alpha 2) were transiently expressed in monkey COS cells under the transcriptional control of the simian virus 40 early promoter. The proteins were labelled with [35S]methionine during a 16 h incubation and proteins secreted by the cells during this period were separated by polyacrylamide gel electrophoresis and subsequently visualized by fluorography. Under the conditions used, the IFNs represented 5 to 10% of the total amount of secreted proteins. All genes were found to encode biologically active IFN subspecies, including alpha 4 which has a deletion of five amino acids. When the specific activities of the proteins were compared, it appeared that the specific antiviral activity of alpha 4 on mouse cells was three- to sixfold higher than the activities of the other natural IFN subspecies. The specific activities of the hybrid proteins were similar to those of the natural proteins, except for the alpha 2 alpha 4 hybrid which had a higher specific activity than the original proteins. The ability of the natural and hybrid subspecies to protect hamster cells against viral infection was determined using MuIFN-alpha 1 as a standard. Large differences in activity were found, with alpha 6 as the most and alpha 4 as the least active subspecies. |
| Databáze: | OpenAIRE |
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