Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran

Autor: Kioomars Soleymani, Aliasghar Farazi, Fereshteh Sadat Seddighinia, Daryoush Hamedi Asl, Saman Soleimanpour, R Keshavarz, Keyvan Tadayon, Nader Mosavari
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Tuberculosis Research and Treatment
Tuberculosis Research and Treatment, Vol 2014 (2014)
ISSN: 2090-1518
2090-150X
Popis: Human tuberculosis caused byMycobacterium tuberculosis(Mtb) remains a significant disease in many countries. According to Iran’s borders with Afghanistan and Pakistan, which are among the 22 high burden countries around the world, this study was conducted to analyze the current molecular epidemiology of tuberculosis and survey genetic diversity ofMtbstrains in Markazi Province in center of Iran. In this experimental study, 75 sputum specimens and one gastric lavage from all smear-positive TB patients admitted to the public hospitals across the Markazi Province were cultured on specific mycobacterial culture media. Genomic DNA was digested byPvuII and transferred to positively charged nylon membrane by southern blotting method and hybridization by PGRS and DR probes. Genotyping of the isolates by PGRS-RFLP and DR-RFLP displayed a wide range of genetic diversity as 25 and 26 genotypes were identified, respectively. Generally speaking, despite the relatively limited number of isolates in the study, high age of patients and also large heterogeneity found in the setting are both in opposition to active circulation ofMtbstrains between patients under study either Iranian or Afghan nationals. Thus, it seems that reactivation of latent infection has had the main role in the spread of tuberculosis.
Databáze: OpenAIRE
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