The formulation of bacteriophage in a semi solid preparation for control of Propionibacterium acnes growth
| Autor: | Zoe A. Dyson, Teagan L. Brown, Robert J. Seviour, Joseph Tucci, Steve Petrovski |
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| Rok vydání: | 2023 |
| Předmět: |
0301 basic medicine
Phage display medicine.medical_treatment viruses Chemistry Pharmaceutical Antibiotics lcsh:Medicine Artificial Gene Amplification and Extension Polymerase Chain Reaction Bacteriophage Phage Display Degree Celsius Medicine and Health Sciences Bacteriophages lcsh:Science Phylogeny Data Management Skin Uncategorized Viral Genomics Multidisciplinary biology Antimicrobials Drugs Genomics Cetomacrogol Molecular Biology Display Techniques Phylogenetics Viruses Research Article Computer and Information Sciences Phage therapy medicine.drug_class 030106 microbiology Molecular Sequence Data Microbial Genomics Dermatology Research and Analysis Methods Microbiology 03 medical and health sciences Propionibacterium acnes Virology Microbial Control medicine Genetics Humans Evolutionary Systematics Molecular Biology Techniques Gene Prediction Molecular Biology Taxonomy Pharmacology Molecular Biology Assays and Analysis Techniques Evolutionary Biology Base Sequence lcsh:R Organisms Biology and Life Sciences Computational Biology Sequence Analysis DNA biology.organism_classification Genome Analysis 030104 developmental biology Acne DNA Viral lcsh:Q Sequence Alignment Bacteria |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 3, p e0151184 (2016) |
| DOI: | 10.26181/22002206 |
| Popis: | Aims To isolate and characterise phage which could lyse P. acnes and to formulate the phage into a delivery form for potential application in topical treatment of acne infection. Methods and Results Using standard phage isolation techniques, ten phage capable of lysing P. acnes were isolated from human skin microflora. Their genomes showed high homology to previously reported P. acnes phage. These phage were formulated into cetomacrogol cream aqueous at a concentration of 2.5x108 PFU per gram, and shown to lyse underlying P. acnes cells grown as lawn cultures. These phage formulations remained active for at least 90 days when stored at four degrees Celsius in a light protected container. Conclusions P. acnes phage formulated into cetomacrogol cream aqueous will lyse surrounding and underlying P. acnes bacteria, and are effective for at least 90 days if stored appropriately. Significance and Impact of the Study There are few reports of phage formulation into semi solid preparations for application as phage therapy. The formulation method described here could potentially be applied topically to treat human acne infections. The potential exists for this model to be extended to other phage applied to treat other bacterial skin infections. |
| Databáze: | OpenAIRE |
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