Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein
| Autor: | Morten Elholm, Jens Knudsen, Gurli Bjerking, Karsten Kristiansen, Susanne Mandrup |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Peroxisome proliferator-activated receptor
Gene Expression Retinoid X receptor Biology Transfection Rats Sprague-Dawley Acyl-CoA-binding protein Genetics Tumor Cells Cultured Animals Electrophoretic mobility shift assay Binding site Promoter Regions Genetic Transcription factor chemistry.chemical_classification Diazepam Binding Inhibitor Binding Sites Promoter General Medicine Phosphoproteins Molecular biology Rats Pyrimidines chemistry Electrophoresis Polyacrylamide Gel Carrier Proteins Diazepam binding inhibitor Transcription Factors |
| Zdroj: | Elholm, M, Bjerking, G, Knudsen, J, Kristiansen, K & Mandrup, S 1996, ' Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein ', Gene, vol. 173, no. 2, pp. 233-238 . https://doi.org/10.1016/0378-1119(96)00213-2 |
| ISSN: | 0378-1119 |
| Popis: | Udgivelsesdato: 1996-Sep-16 Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity. |
| Databáze: | OpenAIRE |
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