Integration of physical, genetic and cytogenetic maps of human chromosome 7: isolation and analysis of yeast artificial chromosome clones for 117 mapped genetic markers
| Autor: | Dale C. Peluso, Valerie V. Braden, Robert S. Fulton, Rose M. Mohr-Tldwell, Eric D. Green, Charles L. Magness, Barbara J. Trask, Jean Welssenbach, Hillary Massa, Jacquelyn R. ldol, Joe Kimura, Alllson M. Wllson |
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| Rok vydání: | 1994 |
| Předmět: |
Genetic Markers
Genetics Chromosome 7 (human) Yeast artificial chromosome congenital hereditary and neonatal diseases and abnormalities Positional cloning Contig Chromosome Mapping hemic and immune systems chemical and pharmacologic phenomena General Medicine Biology Gene mapping Genetic marker hemic and lymphatic diseases Humans Cloning Molecular Chromosome 21 Chromosomes Artificial Yeast Molecular Biology Chromosome 22 Chromosomes Human Pair 7 In Situ Hybridization Fluorescence Genetics (clinical) |
| Zdroj: | Human Molecular Genetics. 3:489-501 |
| ISSN: | 1460-2083 0964-6906 |
| DOI: | 10.1093/hmg/3.3.489 |
| Popis: | An important goal for the human genome project is to assemble fully integrated physical, genetic and cytogenetic maps for each human chromosome. Towards that end, we have isolated yeast artificial chromosome (YAC) clones containing 117 of the 119 genetic markers that constitute a recently constructed, detailed genetic map of human chromosome 7. Analysis of these clones reveals numerous examples where adjacent genetic markers have been physically connected, either in individual YACs or in multi-YAC contigs. At present, the 117 genetic markers are contained in fewer than 80 YAC contigs, with most of these contigs uniquely ordered relative to one another based on the genetic map positions of the corresponding markers. These YACs and YAC contigs are estimated to contain approximately 60-85% of the DNA from human chromosome 7. YACs representing 36 genetic markers were mapped by fluorescence in situ hybridization (FISH) to metaphase chromosomes, allowing assignment of these genetic markers to cytogenetic bands along chromosome 7 and placement of the centromere within the genetic map. Together, these studies provide genetically and cytogenetically anchored YAC clones covering the majority of chromosome 7 that will be useful both for the positional cloning of genes and as a framework for assembling a complete YAC-based physical map of the chromosome. |
| Databáze: | OpenAIRE |
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