Determination of Intestinal Viral Loads and Distribution of Bovine Viral Diarrhea Virus, Classical Swine Fever Virus, and Peste Des Petits Ruminants Virus: A Pilot Study

Autor: Henk J. Wisselink, Joris J. Wijnker, Eline Verheij, Bregtje Smid, Wim H.M. van der Poel, Tinka Jelsma
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Microbiology (medical)
Epidemiology
Bioinformatica & Diermodellen
viruses
Kwantitatieve Veterinaire Epidemiologie
Ileum
Classical swine fever virus
Virus
Article
Muscular layer
Mucosa
Muscular layer/serosa
Submucosa
Bio-informatics & Animal models
medicine
Immunology and Allergy
Epidemiology
Bio-informatics & Animal models
Natural casing
Viral load
Molecular Biology
Host Pathogen Interaction & Diagnostics
Epidemiologie
General Immunology and Microbiology
biology
Bacteriologie
Peste des petits ruminants virus
Quantitative Veterinary Epidemiology
Bacteriology
Bacteriology
Host Pathogen Interaction & Diagnostics
biology.organism_classification
Virology
Host Pathogen Interactie & Diagnostiek
Virology & Molecular Biology
Virologie & Moleculaire Biologie
Intestine
Titer
Infectious Diseases
medicine.anatomical_structure
Classical swine fever
Peste-des-petits-ruminants virus
Epidemiologie
Bioinformatica & Diermodellen
Bacteriologie
Host Pathogen Interactie & Diagnostiek
WIAS
Medicine
Bovine viral diarrhea virus
Zdroj: Pathogens 10 (2021) 9
Pathogens, 10(9)
Pathogens
Volume 10
Issue 9
Pathogens, Vol 10, Iss 1188, p 1188 (2021)
ISSN: 2076-0817
Popis: The aim of this pilot study was to determine viral loads and distribution over the total length, at short distances, and in the separate layers of the intestine of virus-infected animals for future inactivation studies. Two calves, two pigs, and two goats were infected with bovine viral diarrhoea virus (BVDV), classical swine fever virus (CSFV), and peste des petits ruminants virus (PPRV), respectively. Homogenously distributed maximum BVDV viral loads were detected in the ileum of both calves, with a mean titer of 6.0 log10 TCID50-eq/g. The viral loads in colon and caecum were not distributed homogenously. In one pig, evenly distributed CSFV mean viral loads of 4.5 and 4.2 log10 TCID50-eq/g were found in the small and large intestines, respectively. Mucosa, submucosa, and muscular layer/serosa showed mean viral loads of 5.3, 3.4, and 4.0 log10 TCID50-eq/g, respectively. Homogenous distribution of PPRV was shown in the ileum of both goats, with a mean viral load of 4.6 log10 TCID50-eq/g. Mean mucosa, submucosa, and muscular layer/serosa viral loads were 3.5, 2.8, and 1.7 log10 TCID50-eq/g, respectively. This pilot study provides essential data for setting up inactivation experiments with intestines derived from experimentally infected animals, in which the level and the homogeneous distribution of intestinal viral loads are required.
Databáze: OpenAIRE
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