The cell-mediated immune response toBorrelia afzelii,gariniiandburgdorferiin C57BL/6 mice is dependent on antigen specificity
| Autor: | Mojca Škoberne, Igor Gruntar, Vladimir Kotnik, Tadej Malovrh |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Microbiology (medical) Cellular immunity Time Factors Immunology Epitopes T-Lymphocyte Enzyme-Linked Immunosorbent Assay Spirochaetaceae Lymphocyte Activation medicine.disease_cause Borrelia afzelii Microbiology Mice Antigen Antibody Specificity Borrelia parasitic diseases medicine Animals Immunology and Allergy Borrelia burgdorferi Cells Cultured Antigens Bacterial Immunity Cellular biology Immune Sera General Medicine bacterial infections and mycoses biology.organism_classification Virology Mice Inbred C57BL Infectious Diseases biology.protein Female Borrelia garinii Antibody Borrelia Infections Spleen |
| Zdroj: | FEMS Immunology & Medical Microbiology. 28:233-240 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1111/j.1574-695x.2000.tb01482.x |
| Popis: | Inbred C57BL/6 mice were challenged with Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto and tested for antigen-specific T-cell response in vitro. The sonicated preparations of in vitro grown spirochetes were capable of stimulating polyclonal proliferation and specific cell-mediated response, depending on duration of the cell culture. Murine splenocytes previously sensitized to B. burgdorferi sensu lato (s.l. ), but not those from control mice, could be induced for antigen-specific proliferation in vitro. Moreover, detectable cell-mediated response could be induced only with antigen preparations derived from a corresponding strain but not with those obtained from other Borrelia genospecies as revealed by the [(3)H]thymidine incorporation assay. The current study considers that the strict B. burgdorferi s.l. antigen-specific response may also be expected in infections in humans and contributes to the explanation of the frequently poor antibody- and cell-mediated immune response observed in patients diagnosed with Lyme disease. |
| Databáze: | OpenAIRE |
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