FOXO3 Mediates Tooth Movement by Regulating Force-Induced Osteogenesis

Autor:	Yang Yang, Yueyang Hong, Xiangru Huang, Qinggang Dai, Hongyuan Xu, Xinyi Gong, Lingyong Jiang, Anting Jin, Xiaofeng Gao
Rok vydání:	2021
Předmět:	Tooth Movement Techniques biology Chemistry Cellular differentiation Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell biology Bone remodeling RUNX2 Osteogenesis Osteocalcin biology.protein FOXO3 Stress Mechanical Mechanotransduction General Dentistry Transcription factor
Zdroj:	Journal of Dental Research. 101:196-205
ISSN:	1544-0591 0022-0345
DOI:	10.1177/00220345211021534
Popis:	The high prevalence of malocclusion and dentofacial malformations means that the demand for orthodontic treatments has been increasing rapidly. As the biological basis of orthodontic treatment, the mechanism of mechanical force–induced alveolar bone remodeling during orthodontic tooth movement (OTM) has become the key scientific issue of orthodontics. It has been demonstrated that bone mesenchymal stem cells (BMSCs) are crucial for bone remodeling and exhibit mechanical sensing properties. Mechanical force can promote osteoblastic differentiation of BMSCs and osteogenesis, but the key factor that mediates mechanical force–induced osteogenesis during OTM remains unclear. In this study, by performing reverse-phase protein arrays on BMSCs exposed to mechanical force, we found that the expression level of forkhead box O3 (FOXO3) was significantly upregulated during the mechanical force–induced osteoblastic differentiation of BMSCs. The number of FOXO3-positive cells was consistently higher on the OTM side as compared with the control side and accompanied by the enhancement of osteogenesis. Remarkably, inhibiting FOXO3 with repaglinide delayed OTM by severely impairing mechanical force–induced bone formation in vivo. Moreover, knockdown of FOXO3 effectively inhibited the mechanical force–induced osteoblastic differentiation of BMSCs, whereas the overexpression of FOXO3 enhanced this effect. Mechanistically, we revealed a novel regulatory model in which FOXO3 promoted osteocalcin transcription by activating its promoter in cooperation with runt-related transcription factor 2 (RUNX2). We collectively obtained the first evidence that FOXO3 is critical for OTM, where it responds to mechanical force and directly regulates downstream osteoblastic differentiation in an efficient manner.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d6bb9bab0ba6fc54695fac868d49067 https://doi.org/10.1177/00220345211021534 Zobrazit plný text záznamu