Dendrite arborization requires the dynein cofactor NudE
| Autor: | Allison M. Abellaneda, Jill Wildonger, Sihui Z. Yang, Ashley L. Arthur |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Ndel1
Microtubule-associated protein Dynein Golgi Apparatus Biology Bioinformatics Microtubules Animals Genetically Modified symbols.namesake Dendrite (crystal) Microtubule Molecular motor Animals Drosophila Proteins NDEL1 NudE Dyneins Dendrites Cell Biology Golgi apparatus Cell biology Dendrite morphogenesis Drosophila melanogaster Dendrite patterning symbols Drosophila Carrier Proteins Microtubule-Associated Proteins Nde1 Research Article |
| Zdroj: | Journal of Cell Science |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.170316 |
| Popis: | The microtubule-based molecular motor dynein is essential for proper neuronal morphogenesis. Dynein activity is regulated by cofactors, and the role(s) of these cofactors in shaping neuronal structure are still being elucidated. Using Drosophila melanogaster, we reveal that the loss of the dynein cofactor NudE results in abnormal dendrite arborization. Our data show that NudE associates with Golgi outposts, which mediate dendrite branching, suggesting that NudE normally influences dendrite patterning by regulating Golgi outpost transport. Neurons lacking NudE also have increased microtubule dynamics, reflecting a change in microtubule stability that is likely to also contribute to abnormal dendrite growth and branching. These defects in dendritogenesis are rescued by elevating levels of Lis1, another dynein cofactor that interacts with NudE as part of a tripartite complex. Our data further show that the NudE C-terminus is dispensable for dendrite morphogenesis and is likely to modulate NudE activity. We propose that a key function of NudE is to enhance an interaction between Lis1 and dynein that is crucial for motor activity and dendrite architecture. |
| Databáze: | OpenAIRE |
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