Gene expression profiles of lens regeneration and development inXenopus laevis
| Autor: | Erica L. Malloch, Michael W. King, Kimberly J. Perry, Caroline W. Beck, Jason Wever, Lisa Fukui, Jonathan J. Henry, Verity Johnson |
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| Rok vydání: | 2009 |
| Předmět: |
Embryo
Nonmammalian cDNA library Gene Expression Profiling Regeneration (biology) Wnt signaling pathway Xenopus Retinoic acid Gene Expression Regulation Developmental Xenopus Proteins Biology biology.organism_classification Molecular biology Matrix Metalloproteinases Article Hedgehog signaling pathway Cell biology Xenopus laevis chemistry.chemical_compound chemistry Lens Crystalline Gene expression Animals Transcription factor In Situ Hybridization Developmental Biology |
| Zdroj: | Developmental Dynamics. 238:2340-2356 |
| ISSN: | 1097-0177 1058-8388 |
| DOI: | 10.1002/dvdy.21998 |
| Popis: | Seven hundred and thirty-four unique genes were recovered from a cDNA library enriched for genes up-regulated during the process of lens regeneration in the frog Xenopus laevis. The sequences represent transcription factors, proteins involved in RNA synthesis/processing, components of prominent cell signaling pathways, genes involved in protein processing, transport, and degradation (e.g., the ubiquitin/proteasome pathway), matrix metalloproteases (MMPs), as well as many other proteins. The findings implicate specific signal transduction pathways in the process of lens regeneration, including the FGF, TGF-beta, MAPK, Retinoic acid, Wnt, and hedgehog signaling pathways, which are known to play important roles in eye/lens development and regeneration in various systems. In situ hybridization revealed that the majority of genes recovered are expressed during embryogenesis, including in eye tissues. Several novel genes specifically expressed in lenses were identified. The suite of genes was compared to those up-regulated in other regenerating tissues/organisms, and a small degree of overlap was detected. Developmental Dynamics 238:2340–2356, 2009. © 2009 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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