Intraoperative Sentinel Lymph Node Evaluation: Implications of Cytokeratin 19 Expression for the Adoption of OSNA in Oral Squamous Cell Carcinoma
Autor: | Andreas Kjaer, Rehab El Maddani, Richard Shaw, Katalin Kiss, Irene Wessel, Christian von Buchwald, Anders Christensen, Giedrius Lelkaitis, Triantafillos Liloglou, Janet M. Risk, Max Robinson, Kapil Java, Anna Long, Triantafyllou Asterios |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Sentinel lymph node Gene Expression Real-Time Polymerase Chain Reaction Sensitivity and Specificity 03 medical and health sciences Cytokeratin Intraoperative Period 0302 clinical medicine Surgical oncology Carcinoma medicine Humans RNA Messenger False Negative Reactions In Situ Hybridization Neoplasm Staging Mouth neoplasm Keratin-19 business.industry Sentinel Lymph Node Biopsy Nucleic acid amplification technique medicine.disease Immunohistochemistry stomatognathic diseases 030104 developmental biology Oncology 030220 oncology & carcinogenesis Lymphatic Metastasis Carcinoma Squamous Cell Surgery Mouth Neoplasms Lymph Sentinel Lymph Node business Head and Neck Oncology Nucleic Acid Amplification Techniques |
Zdroj: | Annals of Surgical Oncology ANNALS OF SURGICAL ONCOLOGY Shaw, R, Christensen, A, Java, K, Maddani, R E, Liloglou, T, Asterios, T, von Buchwald, C, Wessel, I, Kiss, K, Kjaer, A, Lelkaitis, G, Long, A, Risk, J & Robinson, M 2016, ' Intraoperative Sentinel Lymph Node Evaluation : Implications of Cytokeratin 19 Expression for the Adoption of OSNA in Oral Squamous Cell Carcinoma ', Annals of Surgical Oncology, vol. 23, no. 12, pp. 4042-4048 . https://doi.org/10.1245/s10434-016-5337-6 |
ISSN: | 1534-4681 1068-9265 |
Popis: | Background Intraoperative analysis of sentinel lymph nodes would enhance the care of early-stage oral squamous cell carcinoma (OSCC). We determined the frequency and extent of cytokeratin 19 (CK19) expression in OSCC primary tumours and surrounding tissues to explore the feasibility of a “clinic-ready” intraoperative diagnostic test (one step nucleic acid amplification—OSNA, sysmex). Methods Two cohorts were assembled: cohort 1, OSCC with stage and site that closely match cases suitable for sentinel lymph node biopsy (SLNB); cohort 2, HNSCC with sufficient fresh tumour tissue available for the OSNA assay (>50 mg). CK19 assays included qRT-PCR, RNA in situ hybridisation (ISH), and immunohistochemistry (IHC), as well as OSNA. Results CK19 mRNA expression was detected with variable sensitivity, depending on method, in 60–80% of primary OSCC tumours, while protein expression was observed in only 50% of tumours. Discordance between different techniques indicated that OSNA was more sensitive than qRT-PCR or RNA-ISH, which in turn were more sensitive than IHC. OSNA results showed CK19 expression in 80% of primary cases, so if used for diagnosis of lymph node metastasis would lead to a false-negative result in 20% of patients with cervical lymph node metastases. Conclusions OSNA in its current form is not suitable for use in OSCC SLNB due to inadequate expression of the CK19 target in all case. However, the same assay technology would likely be very promising if applied using a more ubiquitous squamous epithelial target. Electronic supplementary material The online version of this article (doi:10.1245/s10434-016-5337-6) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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