Immune suppression of vaccine-induced CD8⁺ T cell-responses by gamma retrovirus envelope is mediated by interleukin-10 producing CD4⁺ T cells
| Autor: | Philip Podschwadt, Anna Malyshkina, Sonja Windmann, Athanasios Papadamakis, Leonie Kerkmann, Dennis Lapuente, Matthias Tenbusch, Mengji Lu, Michael Schindler, Karl Sebastian Lang, Wiebke Hansen, Wibke Bayer |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Medizinische Fakultät » Universitätsklinikum Essen » Institut für Medizinische Mikrobiologie
Medizinische Fakultät » Universitätsklinikum Essen » Institut für Immunologie Immunology Medizin Immunology and Allergy retrovirus -- envelope -- Env -- Friend virus -- immunosuppression -- interleukin-10 -- IL-10 ddc:610 Medizinische Fakultät » Universitätsklinikum Essen » Institut für Virologie |
| Popis: | Retroviral envelope (Env) proteins have long been recognized to exhibit immunosuppressive properties, which affect the CD8+ T-cell response to an infection but also to immunization. Interestingly, we previously showed in the Friend murine leukemia virus (F-MuLV) model that the surface Env protein gp70 also plays a role in immunosuppression, in addition to the immunosuppressive function attributed to the transmembrane Env protein. We now demonstrate that immunization with F-MuLV Env leads to a significant increase in interleukin-10 (IL-10)-producing CD4+ T cells and that the induction of CD8+ T-cell responses in the presence of Env is rescued if the capacity of CD4+ T cells to produce IL-10 is abrogated, indicating a mechanistic role of IL-10-producing CD4+ T cells in mediating the Env-induced suppression of CD8+ T-cell responses in Env co-immunization. We found that CD8+ T-cell responses against different immunogens are not all equally affected. On the other hand, suppression of immunity was observed not only in co-immunization experiments but also for immune control of subcutaneous tumor growth after an Env immunization. Finally, we show that suppression of CD8+ T cells by the surface Env protein is observed not only for Friend MuLV Env but also for the Env proteins of other gamma retroviruses. Taken together, our results show that IL-10-producing CD4+ T cells mechanistically underlie the Env-mediated suppression of CD8+ T-cell responses and suggest the presence of an immunosuppressive motif in the surface Env protein of gamma retroviruses. |
| Databáze: | OpenAIRE |
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