Arabidopsis RCD1 coordinates chloroplast and mitochondrial functions through interaction with ANAC transcription factors
| Autor: | Julia Krasensky-Wrzaczek, Lauri Nikkanen, Esa Tyystjärvi, Jaakko Kangasjärvi, Nina Sipari, Maarit Hellman, Markku Keinänen, Mikael Brosché, Katrien Van Der Kelen, Saleh Alseekh, Jarkko Salojärvi, Fayezeh Aarabi, Arjun Tiwari, Bert De Rybel, Sari Järvi, Brecht Wybouw, Kerri Hunter, Julia P. Vainonen, Helena Tossavainen, Michael Wrzaczek, Alisdair R. Fernie, Frank Van Breusegem, Eevi Rintamäki, Eva-Mari Aro, Perttu Permi, Alexey Shapiguzov |
|---|---|
| Přispěvatelé: | Plant ROS-Signalling, Plant Biology, Organismal and Evolutionary Biology Research Programme, Viikki Plant Science Centre (ViPS), Receptor-Ligand Signaling Group, Institute of Biotechnology, Bioinformatics for Molecular Biology and Genomics (BMBG), Plant stress and natural variation, Perttu Permi / Principal Investigator, School of Biological Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine retrograde signaling Chloroplasts Arabidopsis Plant Biology Mitochondrion 01 natural sciences Electron Transport Complex III Gene Expression Regulation Plant OXIDATIVE STRESS-RESPONSE Transcriptional regulation CYCLIC ELECTRON FLOW Biology (General) Nuclear protein ANAC transcription factors 1183 Plant biology microbiology virology reactive oxygen species biology Chemistry RETROGRADE REGULATION General Neuroscience Nuclear Proteins food and beverages General Medicine Plants Genetically Modified Science::Biological sciences [DRNTU] Cell biology Mitochondria Chloroplast viherhiukkaset Medicine Signal transduction mitochondrial functions Research Article Signal Transduction QH301-705.5 Science mitokondriot Genetics and Molecular Biology General Biochemistry Genetics and Molecular Biology PROTEIN COMPLEXES SIGNALING PATHWAYS 03 medical and health sciences chloroplast Stress Physiological ALTERNATIVE OXIDASES kasvit ENZYME-ACTIVITIES redox signaling Transcription factor arabidopsis RCD1 General Immunology and Microbiology biokemia Arabidopsis Proteins ta1182 Biology and Life Sciences biology.organism_classification 030104 developmental biology CELL-DEATH PLANT-MITOCHONDRIA A. thaliana General Biochemistry Retrograde signaling GENES-ENCODING MITOCHONDRIAL proteiinit 010606 plant biology & botany Transcription Factors |
| Zdroj: | ELIFE eLife eLife, Vol 8 (2019) |
| ISSN: | 2050-084X |
| Popis: | Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo and suppresses the activity of the transcription factors ANAC013 and ANAC017, which mediate a ROS-related retrograde signal originating from mitochondrial complex III. Inactivation of RCD1 leads to increased expression of mitochondrial dysfunction stimulon (MDS) genes regulated by ANAC013 and ANAC017. Accumulating MDS gene products, including alternative oxidases (AOXs), affect redox status of the chloroplasts, leading to changes in chloroplast ROS processing and increased protection of photosynthetic apparatus. ROS alter the abundance, thiol redox state and oligomerization of the RCD1 protein in vivo, providing feedback control on its function. RCD1-dependent regulation is linked to chloroplast signaling by 3'-phosphoadenosine 5'-phosphate (PAP). Thus, RCD1 integrates organellar signaling from chloroplasts and mitochondria to establish transcriptional control over the metabolic processes in both organelles. eLife digest Most plant cells contain two types of compartments, the mitochondria and the chloroplasts, which work together to supply the chemical energy required by life processes. Genes located in another part of the cell, the nucleus, encode for the majority of the proteins found in these compartments. At any given time, the mitochondria and the chloroplasts send specific, ‘retrograde’ signals to the nucleus to turn on or off the genes they need. For example, mitochondria produce molecules known as reactive oxygen species (ROS) if they are having problems generating energy. These molecules activate several regulatory proteins that move into the nucleus and switch on MDS genes, a set of genes which helps to repair the mitochondria. Chloroplasts also produce ROS that can act as retrograde signals. It is still unclear how the nucleus integrates signals from both chloroplasts and mitochondria to ‘decide’ which genes to switch on, but a protein called RCD1 may play a role in this process. Indeed, previous studies have found that Arabidopsis plants that lack RCD1 have defects in both their mitochondria and chloroplasts. In these mutant plants, the MDS genes are constantly active and the chloroplasts have problems making ROS. To investigate this further, Shapiguzov, Vainonen et al. use biochemical and genetic approaches to study RCD1 in Arabidopsis. The experiments confirm that this protein allows a dialog to take place between the retrograde signals of both mitochondria and chloroplasts. On one hand, RCD1 binds to and inhibits the regulatory proteins that usually activate the MDS genes under the control of mitochondria. This explains why, in the absence of RCD1, the MDS genes are always active, which is ultimately disturbing how these compartments work. On the other hand, RCD1 is also found to be sensitive to the ROS that chloroplasts produce. This means that chloroplasts may be able to affect when mitochondria generate energy by regulating the protein. Finally, further experiments show that MDS genes can affect both mitochondria and chloroplasts: by influencing how these genes are regulated, RCD1 therefore acts on the two types of compartments. Overall, the work by Shapiguzov, Vainonen et al. describes a new way Arabidopsis coordinates its mitochondria and chloroplasts. Further studies will improve our understanding of how plants regulate these compartments in different environments to produce the energy they need. In practice, this may also help plant breeders create new varieties of crops that produce energy more efficiently and which better resist to stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|