Isolation and genomic characterization of the ibuprofen-degrading bacterium Sphingomonas strain MPO218
| Autor: | Antonio J. Pérez-Pulido, Magaly Aulestia, Amando Flores, Eduardo Santero, Eva María Camacho, Eugenio L. Mangas |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2021 |
| Předmět: |
Gene Transfer
Horizontal Microorganism Ibuprofen Sphingomonas Microbiology Water Purification 03 medical and health sciences Plasmid Water Pollution Chemical Sphingopyxis granuli Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences biology 030306 microbiology Genomics biology.organism_classification Sphingomonadaceae Biodegradation Environmental Biochemistry Sphingomonas wittichii Horizontal gene transfer Energy source Water Pollutants Chemical Bacteria Plasmids |
| Zdroj: | Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | The presence of pharmaceutical compounds in waters and soils is of particular concern because these compounds can be biologically active, even at environmental concentrations. Most pharmaceutical contaminants result from inefficient removal of these compounds during wastewater treatment. Although microorganisms able to biodegrade pharmaceuticals compounds have been described, the isolation and characterization of new bacterial strains capable of degrading drugs remain important to improve the removal of this pollutant. In this work, we describe the Sphingomonas wittichii strain MPO218 as able to use ibuprofen as the sole carbon and energy source. The genome of MPO218 consists of a circular chromosome and two circular plasmids. Our analysis shows that the largest plasmid, named pIBU218, is conjugative and can horizontally transfer the capability of growing on ibuprofen after conjugation with another related bacterium, Sphingopyxis granuli TFA. This plasmid appears to be unstable since it undergoes different deletions in absence of selection when growth on ibuprofen is not selected. This is the first described example of a natural and conjugative plasmid that enables growth on ibuprofen and is another example of how horizontal gene transfer plays a crucial role in the evolution of bacteria. This work was supported by the Spanish Ministry of Science, Innovation and Universities grant BIO2014-57545-R. |
| Databáze: | OpenAIRE |
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