IgM Anamnestic Immune Response to the Haptenic Determinant DNP on a Thymus-Independent Carrier

Autor:	P, Del Guercio, N, Thobie, M F, Poirier
Rok vydání:	1974
Předmět:	Male B-Lymphocytes Immune Sera Immunology Immunization Secondary Iodoacetates Coliphages Mice Immunoglobulin M Radiation Chimera Antibody Formation Mice Inbred CBA Animals Immunology and Allergy Carrier Proteins Immunologic Memory Dinitrophenols Spleen Mercaptoethanol
Zdroj:	The Journal of Immunology. 112:427-429
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.112.1.427
Popis:	Antibody formation in mice against a haptenic determinant such as dinitrophenyl (DNP),3 is strictly related to the immunogenicity and nature of the carrier. Thus, a carrier that interacts only with non-thymus-derived (B) lymphocytes, e.g., levan (LE) (1), induces in vivo a thymus-independent primary response to DNP (2). The DNP-LE conjugate produces only IgM antibody against DNP (2), and the response is not potentiated by Freund's adjuvants (3). This report describes the anamnestic response to the DNP-LE conjugate. LE with the reduced molecular weight of 100,000 was coupled to DNP, as previously described (2). Male CBA mice (20 to 25 g) were injected i.v. with different doses of DNP6-LE. Sixteen weeks later mice were challenged i.v. with 10 µg of DNP6-LE. In transfer experiments CBA mice were primed i.v. with 100 µg of DNP6-LE, and 1 or 4 weeks later their spleens were removed and homogenized in medium 199 (4).
Databáze:	OpenAIRE
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