Transmembrane exchange of fluorosugars: Characterization of red cell GLUT1 kinetics using 19F NMR
| Autor: | Bruno Linclau, Ilya Kuprov, Philip W. Kuchel, Clement Q. Fontenelle, Dmitry Shishmarev |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Membrane permeability biology Chemistry Kinetics Biophysics Glucose transporter Nuclear magnetic resonance spectroscopy Fluorine-19 NMR Membrane transport Transmembrane protein 03 medical and health sciences 030104 developmental biology biology.protein GLUT1 Two-dimensional nuclear magnetic resonance spectroscopy |
| Popis: | We developed a novel approach for quantifying the equilibrium-exchange kinetics of carrier-mediated transmembrane transport of fluorinated substrates. Our method is based on an adapted kinetic theory describing the concentration dependence of the transmembrane-exchange rates of two simultaneously transported species. Using the new approach, we quantified the kinetics of membrane transport of both anomers of three monofluorinated glucose analogues in human erythrocytes (red blood cells: RBCs) using 19F nuclear magnetic resonance (NMR) exchange spectroscopy (EXSY). An inosine-based glucose-free medium was shown to promote survival and stable metabolism of RBCs over the duration of the experiments (a few hours). Earlier NMR studies only yielded the apparent rate constants and transmembrane fluxes of the anomeric species, whereas we were able to categorize the two anomers in terms of the catalytic activity (specificity constants) of the glucose transport protein GLUT1 towards them. Differences in the membrane permeability of the three glucose analogues were qualitatively interpreted in terms of local perturbations in the bonding of substrates to amino-acid residues in the active site of GLUT1. The methodology of this work will be applicable to studies of other carrier-mediated membrane transport processes, especially those with competition between simultaneously transported species. The GLUT1-specific results will apply to the design of probes of glucose transport, or inhibitors of glucose metabolism in cells including those exhibiting the Warburg effect.ABBREVIATIONSEXSYexchange spectroscopyFDGfluoro-deoxy-glucoseFDG-nn-fluoro-n- deoxy-D-glucose (n = 2, 3, 4)FIDfree induction decayGlcD-glucoseNMRnuclear magnetic resonanceRBCred blood cell |
| Databáze: | OpenAIRE |
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