Additional file 1: of Soluble epoxide hydrolase modulates immune responses in activated astrocytes involving regulation of STAT3 activity

Autor: Hung, Chia-Chi, Yi-Hsuan Lee, Kuo, Yi-Min, Pei-Chien Hsu, Huey-Jen Tsay, Ying-Ting Hsu, Chih-Chin Lee, Liang, Jia-Jun, Feng-Shiun Shie
Rok vydání: 2019
Předmět:
DOI: 10.6084/m9.figshare.8246579.v1
Popis: Figure S1. The expression levels of sEH by genetic manipulations in astrocytes. The expression levels of sEH in primary astrocytes derived from Wt mice were genetically manipulated by using commercially available reagents for the RNA silencing of sEH (si-sEH) and the overexpression of sEH (over-sEH). The levels of sEH mRNA in si-sEH and over-sEH astrocytes were 28 ± 3% (n = 9) and 5.8 ± 1.2 × 106% (n = 6) of the control, respectively, as measured by qPCR (Additional file 1: Figure S1A, B). The mRNA levels of sEH in non-transfected primary astrocytes appeared to be downregulated by LPS treatments (38 ± 5% of the control, n = 7, p
Databáze: OpenAIRE