Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
| Autor: | Rami S. Kantar, Elie Tabet, Bakhos A. Tannous, Ghazal Lashgari, Grant K. Lewandrowski, Litia Carvalho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Tivozanib Treatment outcome Angiogenesis Inhibitors Article Copepoda 03 medical and health sciences Gaussia chemistry.chemical_compound Cell Line Tumor Neoplasms Coelenterazine medicine Animals Humans Bioluminescence imaging Luciferase Luciferases Luminescent Agents Multidisciplinary biology Phenylurea Compounds Anti angiogenic Imidazoles biology.organism_classification Recombinant Proteins 3. Good health Treatment Outcome 030104 developmental biology chemistry Pyrazines Tumor vascularity Luminescent Measurements Quinolines Neoplasm Transplantation medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep26353 |
| Popis: | We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was possible. We applied this method for longitudinal monitoring of tumor vascularity in response to the anti-angiogenic drug tivozanib. This simple and sensitive method could be extended to image blood vessels/vasculature in many different fields. |
| Databáze: | OpenAIRE |
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