Comparison of the mode of action of ras p21 with those of protein kinases A and C in the stimulation of gene expression in NIH/3T3 cells
| Autor: | Yuichi Hori, Kozo Kaibuchi, Naohisa Oku, Yasuo Fukumoto, Yoshimi Takai, Hiroyuki Fujioka |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
viruses Recombinant Fusion Proteins Biophysics ras p21 Oncogene Protein p21(ras) Transfection Biochemistry Cell Line Mice Structural Biology Complementary DNA Proto-Oncogene Proteins Genetics Animals NCK1 NIH/3T3 cell Protein kinases A and C Enhancer Luciferases Promoter Regions Genetic Molecular Biology Gene Protein Kinase C Regulation of gene expression Rous sarcoma virus biology Kinase Cell Biology biology.organism_classification Molecular biology Enhancer/promoter Enhancer Elements Genetic Gene Expression Regulation Metallothionein Protein Kinases Proto-Oncogene Proteins c-fos |
| Zdroj: | FEBS letters. 269(1) |
| ISSN: | 0014-5793 |
| Popis: | To compare the mode of action of ras p21 with those of protein kinases A and C in the regulation of gene expression in NIH/3T3 cells, we investigated the transcriptional activity of various enhancer/promoters and enhancer motifs in the cells transfected with the c-Ha- ras val12 complementary DNA (cDNA). The results indicate that the c-Ha- ras val12 protein stimulates the enhancer/promoters of the c-fos gene, the metallothionein II A gene, the simian virus 40 (SV40) virus genome and the Rous sarcoma (RS) virus genome, and the serum-response element and the 12- O -tetradecanoylphorbol-13-acetate (TPA)-response element in a manner independent of protein kinases A and C in NIH/3T3 cells. |
| Databáze: | OpenAIRE |
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