Stage-specific expression of an odorant receptor underlies olfactory behavioral plasticity in Spodoptera littoralis larvae

Autor: Lucie Conchou, Peter J. Anderson, Vito Antonio Giannuzzi, Valeria Rossi, Eric Conti, Fotini Koutroumpa, Santosh Revadi, Gert Martin Hunger, Gabriele Rondoni, Emmanuelle Jacquin-Joly, William B. Walker, Paul G. Becher
Přispěvatelé: Swedish University of Agricultural Sciences (SLU), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Dept. of Pharmaceutical Sciences, University of Perugia, Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-16-CE21-0002,DEMETER,Bio-olfacticides: produire plus avec moins d'insecticides(2016)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
Olfactory system
animal structures
QH301-705.5
Physiology
Foraging
Sensory system
Plant Science
Olfaction
Spodoptera
Receptors
Odorant
010603 evolutionary biology
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Structural Biology
Receptors
Animals
Biology (General)
Spodoptera littoralis
Sensory cue
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Modulation
0303 health sciences
Larval transcriptome
biology
α-Humulene
fungi
Cell Biology
biology.organism_classification
Cell biology
Smell
Electrophysiology
Odorant
Olfactometer
Larva
[SDE]Environmental Sciences
Instar
β-Caryophyllene
CRISPR-Cas9
General Agricultural and Biological Sciences
Odorant receptor
Research Article
Developmental Biology
Biotechnology
Zdroj: BMC Biology
BMC Biology, BioMed Central, 2021, 19 (1), ⟨10.1186/s12915-021-01159-1⟩
BMC Biology, Vol 19, Iss 1, Pp 1-18 (2021)
BMC Biology, 2021, 19 (1), pp.231. ⟨10.1186/s12915-021-01159-1⟩
ISSN: 1741-7007
DOI: 10.1186/s12915-021-01159-1⟩
Popis: Background The detection of environmental cues and signals via the sensory system directs behavioral choices in diverse organisms. Insect larvae rely on input from the chemosensory system, mainly olfaction, for locating food sources. In several lepidopteran species, foraging behavior and food preferences change across larval instars; however, the molecular mechanisms underlying such behavioral plasticity during larval development are not fully understood. Here, we hypothesize that expression patterns of odorant receptors (ORs) change during development, as a possible mechanism influencing instar-specific olfactory-guided behavior and food preferences. Results We investigated the expression patterns of ORs in larvae of the cotton leafworm Spodoptera littoralis between the first and fourth instar and revealed that some of the ORs show instar-specific expression. We functionally characterized one OR expressed in the first instar, SlitOR40, as responding to the plant volatile, β-caryophyllene and its isomer α-humulene. In agreement with the proposed hypothesis, we showed that first but not fourth instar larvae responded behaviorally to β-caryophyllene and α-humulene. Moreover, knocking out this odorant receptor via CRISPR-Cas9, we confirmed that instar-specific responses towards its cognate ligands rely on the expression of SlitOR40. Conclusion Our results provide evidence that larvae of S. littoralis change their peripheral olfactory system during development. Furthermore, our data demonstrate an unprecedented instar-specific behavioral plasticity mediated by an OR, and knocking out this OR disrupts larval behavioral plasticity. The ecological relevance of such behavioral plasticity for S. littoralis remains to be elucidated, but our results demonstrate an olfactory mechanism underlying this plasticity in foraging behavior during larval development.
Databáze: OpenAIRE
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