Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma
| Autor: | Jacek Kudelski, Marta Bruczko-Goralewska, Grzegorz Młynarczyk, Barbara Darewicz, Lech Romanowicz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Gene Expression Matrix metalloproteinase Pathology and Forensic Medicine 03 medical and health sciences Hydroxyproline chemistry.chemical_compound 0302 clinical medicine Internal medicine Matrix Metalloproteinase 13 medicine Humans Molecular Biology Carcinoma Renal Cell Aged chemistry.chemical_classification Kidney Chemistry Cancer Cell Biology General Medicine Middle Aged medicine.disease Kidney Neoplasms Blot medicine.anatomical_structure Endocrinology Enzyme 030220 oncology & carcinogenesis Collagenase Female Collagen Matrix Metalloproteinase 1 Kidney cancer 030215 immunology medicine.drug |
| Zdroj: | Pathobiology : journal of immunopathology, molecular and cellular biology. 86(4) |
| ISSN: | 1423-0291 |
| Popis: | Background: Collagenases are enzymes starting collagen degradation. The role of collagenases in renal carcinoma development is not well understood. Objective: Evaluation of collagen content and collagenase expression and activity in human kidney cancers. Methods: Collagen content was measured by the hydroxyproline assay. The expression and the content of collagenases were evaluated by Western blotting and ELISA. Fluorogenic substrate was used to measure enzyme activity. Results: Collagen content significantly decreases with the progression of kidney cancer. Both collagenases are first present in high molecular complexes in both control and cancer tissue. The healthy part of the kidney contains similar amounts of both collagenases. Collagenase content decreased significantly in tumor tissue with increasing cancer stage. MMP-13 activity is much higher than that of MMP-1 in all tissues investigated. We observed increasing collagenase activity (MMP-1 and MMP-13) with increasing renal cancer grade. Conclusions: The lower content and higher activity of the collagenases investigated in cancer tissue indicate that most of these enzymes are in active form in renal carcinoma. The lower collagen content in cancer tissue can be explained at least in part by increased collagenase activity. |
| Databáze: | OpenAIRE |
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