Definitive profiling of plasma bile acids as potential biomarkers for human liver diseases using UPLC–HRMS
Autor: | Michael Van Parys, Xiaorong Liang, Dennis L. Miller, Brian Dean, Yang Gao, Yao Shi, Dennis Milanowski |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Clinical Biochemistry Pharmacology Sensitivity and Specificity 01 natural sciences High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Bile Acids and Salts 03 medical and health sciences Humans Medicine General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Human liver business.industry Liver Diseases Liver and kidney 010401 analytical chemistry Reproducibility of Results General Medicine Reference Standards 0104 chemical sciences Clinical Practice Medical Laboratory Technology 030104 developmental biology Liver Drug development Human plasma Potential biomarkers Calibration Biomarker (medicine) business Biomarkers |
Zdroj: | Bioanalysis. 10:917-932 |
ISSN: | 1757-6199 1757-6180 |
Popis: | Aim: Investigation of bile acids (BAs) as biomarkers for liver and kidney diseases has gained momentum recently to fulfill the needs in drug development and clinical practice, but a thorough and rapid profiling of BAs in human plasma has been hindered by the large interindividual variability and lack of selective methods. Results: A selective and efficient UPLC-high resolution mass spectrometry method was developed and fully validated for the definitive profiling of 26 BAs in human plasma with a curve rage of 1–1000 ng/ml and a runtime of 7.2 min. Conclusion: Four BA combinations with good sensitivity and specificity show potential biomarker applications for liver injury and diseases. |
Databáze: | OpenAIRE |
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