Structural and functional homology among chicken, duck, goose, turkey and pigeon interleukin-8 proteins
| Autor: | Yung Fu Wu, Hsien Hseng Yin, Jui Huang Shien, Long Huw Lee, Shiou Her Chiow |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Sequence analysis
Immunology Blotting Western Molecular Sequence Data Heterologous Enzyme-Linked Immunosorbent Assay Molecular cloning Polymerase Chain Reaction Homology (biology) Poultry Goose biology.animal Animals Amino Acid Sequence Cloning Molecular Columbidae Phylogeny chemistry.chemical_classification Antiserum Genetics General Veterinary biology Phylogenetic tree Base Sequence Chemotaxis Interleukin-8 DNA Molecular biology Recombinant Proteins Amino acid chemistry Sequence Alignment |
| Zdroj: | Veterinary immunology and immunopathology. 125(3-4) |
| ISSN: | 0165-2427 |
| Popis: | Interleukin (IL)-8-encoding regions of five avian species were cloned, sequenced and characterized. Each IL-8-encoding region is 312 nucleotides long and encodes IL-8 which is 103 amino acids. Pairwise sequence analysis showed that sequence identities of IL-8-encoding regions ranged from 87% to 100%. The IL-8 protein identities varied from 84% to 100%. Phylogenetic analysis indicated that IL-8-encoding regions and encoded proteins of chicken, duck, goose and turkey clustered together and evolved into a distinct phylogenetic lineage from that of pigeon which evolved into a second lineage. The results from binding reactivities of antiserum against each recombinant IL-8 (rIL-8) protein to homologous or heterologous rIL-8 proteins, chemotactic activities of each rIL-8 protein or reduction levels of the chemotactic activity of rIL-8 protein which was pretreated with homologous or heterlogous antiserum have suggested that all five IL-8 proteins were functionally active, and shared structural and functional identity with each other. |
| Databáze: | OpenAIRE |
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