Biological activities of Cu(II) and Hg(II) complexes of a heptadentate Schiff base ligand
| Autor: | Emel Ergene, Hülya Sivas, Kadriye Benkli |
|---|---|
| Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Sivas, Hülya, Benkli, Kadriye |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Mtt
endocrine system Schiff base Physiology Chemistry Ligand Stereochemistry Acridine orange Metal Complexes Apoptosis Cell Biology Key words: Metal complexes apoptosis DNA fragmentation cancer cells MTT Microbiology Medicinal chemistry Cancer Cells chemistry.chemical_compound Perchlorate Dna Fragmentation Bromide Pyridine Genetics DNA fragmentation General Agricultural and Biological Sciences Cytotoxicity Molecular Biology |
| Zdroj: | Volume: 34, Issue: 4 379-387 Turkish Journal of Biology |
| ISSN: | 0002-8517 1300-0152 1303-6092 |
| Popis: | WOS: 000285179000005 Two metal complexes were synthesized as [Hg(L)](ClO4)(2) and [Cu(L)](ClO4), by the template reaction between 2,6-bis(2-aminothiophenoxymethyl)pyridine and 2,2'-bipyridine-6,6'-dicarboxaldehyde in the presence of Hg(II) and Cu(II) perchlorate salts. The structures of the compounds were elucidated by IR, H-1-NMR, MASS, and Elemental Analyses values. Cytotoxicity of the compounds were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay in normal and cancerous rat fibroblasts. Both compounds showed cytotoxicity on 2 cell lines as a dose-dependent manner. Compound [Hg(L)](ClO4)(2) was more cytotoxic than [Cu(L)](ClO4)(2). Apoptotic activity of compounds was evaluated by acridine orange staining and DNA fragmentation assay. Both cell lines exposed to the compounds exhibited condensed chromatin and appearance of apoptotic bodies. The percentages of all these abnormalities were found to be very high level in ras-transformed 5RP7 fibroblasts. The effect of [Hg(L)](ClO4)(2) was again more stronger than the compound [Cu(L)](ClO4)(2). Although the compound [Cu(L)](ClO4)(2) induced the formation of DNA fragmentation in both cell lines, [Hg(L)](ClO4)(2) induced the DNA fragmentation only in cancerous 5RP7 cells, indicating specific activity. In conclusion, we suggest that both complexes, especially the one with Hg(II) according to its significant differences of apoptotic morphology and DNA fragmentation, exhibit promising potentials as anticancer compounds. |
| Databáze: | OpenAIRE |
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