Nanoparticles-based nanochannels assembled on a plastic flexible substrate for label-free immunosensing
| Autor: | Marisol Espinoza-Castañeda, Alfredo de la Escosura-Muñiz, Madoka Hasegawa, Arben Merkoçi, Laetitia Philippe |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Educación (España) |
| Rok vydání: | 2014 |
| Předmět: |
Analyte
Dip-coating Materials science Label-free immunosensing Nanochannel Substrate (chemistry) Nanoparticle Nanotechnology Electrochemical biosensor Condensed Matter Physics Atomic and Molecular Physics and Optics 3. Good health Indium tin oxide chemistry.chemical_compound chemistry Monolayer Polyethylene terephthalate General Materials Science Polystyrene Electrical and Electronic Engineering |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1998-0000 1998-0124 |
| DOI: | 10.1007/s12274-014-0598-5 |
| Popis: | A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is presented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres (PS, 200 and 500 nm-sized) onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate (ITO/PET) electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe(CN)6]4− flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G (IgG) (chosen as a model analyte) to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems. We acknowledge MINECO (Madrid) for the project MAT2011-25870 and the EU’s support under FP7 contract number 246513 ‘‘NADINE”. Marisol EspinozaCastañeda acknowledges the Ministerio de Educación del Programa de Formación de Profesorado Universitario AP2010-5942. |
| Databáze: | OpenAIRE |
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