The Influence of IL-10 and TNFα on Chondrogenesis of Human Mesenchymal Stromal Cells in Three-Dimensional Cultures
| Autor: | Gundula Schulze-Tanzil, Michal Jagielski, Carola Meier, Anna Völker, Marion Lemke, Wolfgang Ertel, Ulrike Marzahn, Stephan Arens, Johannes Wolf, Owen Godkin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cellular differentiation
Type II collagen 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit bone-marrow MSC Catalysis Article Inorganic Chemistry lcsh:Chemistry articular chondrocytes Chondrocytes three-dimensional culture chondrogenesis Articular cartilage repair TNFα Humans Aggrecans Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Collagen Type II Spectroscopy Aggrecan Cells Cultured Cluster of differentiation Chemistry Tumor Necrosis Factor-alpha Organic Chemistry Mesenchymal stem cell Mesenchymal Stem Cells SOX9 Transcription Factor General Medicine Chondrogenesis Computer Science Applications Cell biology Interleukin-10 Interleukin 10 lcsh:Biology (General) lcsh:QD1-999 Immunology IL-10 Polyglycolic Acid |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 15; Issue 9; Pages: 15821-15844 International Journal of Molecular Sciences, Vol 15, Iss 9, Pp 15821-15844 (2014) |
| ISSN: | 1422-0067 |
| Popis: | Chondrogenic differentiated mesenchymal stromal cells (MSCs) are a promising cell source for articular cartilage repair. This study was undertaken to determine the effectiveness of two three-dimensional (3D) culture systems for chondrogenic MSC differentiation in comparison to primary chondrocytes and to assess the effect of Interleukin (IL)-10 and Tumor Necrosis Factor (TNF)α on chondrogenesis by MSCs in 3D high-density (H-D) culture. MSCs were isolated from femur spongiosa, characterized using a set of typical markers and introduced in scaffold-free H-D cultures or non-woven polyglycolic acid (PGA) scaffolds for chondrogenic differentiation. H-D cultures were stimulated with recombinant IL-10, TNFα, TNFα + IL-10 or remained untreated. Gene and protein expression of type II collagen, aggrecan, sox9 and TNFα were examined. MSCs expressed typical cell surface markers and revealed multipotency. Chondrogenic differentiated cells expressed cartilage-specific markers in both culture systems but to a lower extent when compared with articular chondrocytes. Chondrogenesis was more pronounced in PGA compared with H-D culture. IL-10 and/or TNFα did not impair the chondrogenic differentiation of MSCs. Moreover, in most of the investigated samples, despite not reaching significance level, IL-10 had a stimulatory effect on the type II collagen, aggrecan and TNFα expression when compared with the respective controls. |
| Databáze: | OpenAIRE |
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