Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli
| Autor: | Stefan Behr, Luitpold Fried, Kirsten Jung |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Genotype Histidine Kinase DNA Footprinting Biology medicine.disease_cause Microbiology Plasmid Escherichia coli medicine Deoxyribonuclease I Direct repeat Promoter Regions Genetic Pyruvates Molecular Biology Gene Binding Sites Membrane transport protein Escherichia coli Proteins Histidine kinase Membrane Transport Proteins Gene Expression Regulation Bacterial Articles beta-Galactosidase Major facilitator superfamily Response regulator Biochemistry biology.protein Protein Kinases Gene Deletion Plasmids Protein Binding Transcription Factors |
| Zdroj: | Journal of Bacteriology. 195:807-815 |
| ISSN: | 0021-9193 |
| Popis: | Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overproducing either YpdB or a control protein. Expression levels of 15 genes differed by more than 1.9-fold between the two strains. A comprehensive evaluation of these genes identified yhjX as the sole target of YpdB. Electrophoretic mobility shift assays with purified YpdB confirmed its interaction with the yhjX promoter. Specifically, YpdB binds to two direct repeats of the motif GGCATTTCAT separated by an 11-bp spacer in the yhjX promoter. yhjX encodes a cytoplasmic membrane protein of unknown function that belongs to the major facilitator superfamily of transporters. Finally, we characterized the pattern of yhjX expression and identified extracellular pyruvate as a stimulus for the YpdA/YpdB system. It is suggested that YpdA/YpdB contributes to nutrient scavenging before entry into stationary phase. |
| Databáze: | OpenAIRE |
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