Surfactant Protein A-Producing Cells in Human Fetal Lung Are Good Targets for Recombinant Adenovirus-Mediated Gene Transfer
| Autor: | Elisa Messina, Antonio Procopio, Mariateresa Giuliano, Judith Mühlhauser, Guido Morgese, Assunta Pandolfi |
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| Rok vydání: | 1996 |
| Předmět: |
Fetal Proteins
Pulmonary Surfactant-Associated Proteins Proteolipids Recombinant Fusion Proteins Genetic Vectors Biology Virus Replication medicine.disease_cause law.invention Pulmonary surfactant Genes Reporter law Escherichia coli medicine Humans Lung Pulmonary Surfactant-Associated Protein A Adenoviruses Human Genetic transfer Gene Transfer Techniques Infant Newborn Pulmonary Surfactants Genetic Therapy respiratory system In vitro Surfactant protein A Cell biology Adenoviridae Fetal Diseases Lac Operon Evaluation Studies as Topic Cell culture Pediatrics Perinatology and Child Health Immunology biology.protein Recombinant DNA Protein A |
| Zdroj: | Pediatric Research. 40:142-147 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-199607000-00024 |
| Popis: | Local delivery of Escherichia coli beta-galactosidase gene (beta-gal) to surfactant protein-A (SP-A)-producing cells by a replication-defective recombinant adenovirus (AdCMV.beta-gal) was tested in human 8-12-wk-old fetal lung explants cultured in Waymouth's medium. In contrast to uninfected explants, direct addition of 0.8-1.6 x 10(6) plaque-forming units of AdCMV.beta-gal resulted in beta-galactosidase (beta-Gal)-specific staining of the pulmonary epithelium. SP-A localization by indirect immunofluorescence showed positive specific staining of the beta-Gal+ lung epithelial cells, demonstrating that recombinant-defective adenoviruses efficiently transfer reporter genes to fetal lung SP-A+ cells. The reporter gene expression in SPA+ cells persisted for more than 1 mo. No apparent alteration of morphology, phenotype, and growth was observed. The in vitro human lung model described may be useful for testing DNA constructs for vector-mediated gene therapy, as an approach to the treatment of congenital defects and neonatal disorders, such as respiratory distress syndrome and bronchopulmonary dysplasia. |
| Databáze: | OpenAIRE |
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