| Popis: |
A linkage map, primarily based on SSCP-SNP markers, was constructed using 188 F2:3mapping population progenies produced from a cross between two pearl millet inbred lines having diverse parentage. The skeleton linkage map covered 1019 cM and it comprised of 44 markers distributed across the seven linkage groups. Average adjacent-marker intervals ranged from 14 cM on LG1 to 38 cM on LG6, with an overall mean of 23 cM. Using the F2linkage map and phenotypic data from the F2and F2:3generations of the mapping population, a total of 18 putative QTLs were detected for the three sink-size components. Eight QTLs explained 42.7% of observed phenotypic variation for panicle length using the F2:3data set. For panicle diameter, 5 QTLs explained 45.8% of observed phenotypic variation. Similarly for grain size, 5 QTLs explained 29.6% of phenotypic variation. Genomic regions associated with panicle length, panicle diameter, and grain size were comapped on LG6 between Xpsms88 and Xpsms2270, indicating the existence of a gene or gene cluster. The QTLs for panicle length on LG2 and LG6 (LOD>3in both F2and F2:3data sets), for panicle diameter on LG2 and LG3 (LOD>14in the F2:3data set), and for grain size on LG3 and LG6 (LOD>3in both F2and F2:3data sets) were identified as promising candidates for validation prior to possible application in marker-assisted breeding. |
