Neural labeling and manipulation by neonatal intraventricular viral injection in mice
| Autor: | Mengzhe Wang, Thomas Misgeld, Monika S. Brill |
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| Rok vydání: | 2022 |
| Předmět: |
Neurons
Microscopy Science (General) General Immunology and Microbiology viruses General Neuroscience Genetic Vectors Biotechnology and bioengineering Gene Expression Dependovirus General Biochemistry Genetics and Molecular Biology genetics [Dependovirus] Injections Q1-390 Mice Model Organisms metabolism [Neurons] Developmental biology Protocol Genetics Animals ddc:600 Molecular Biology Injections Intraventricular Neuroscience |
| Zdroj: | STAR Protocols 3(1), 101081 (2022). doi:10.1016/j.xpro.2021.101081 STAR Protocols STAR Protocols, Vol 3, Iss 1, Pp 101081-(2022) |
| ISSN: | 2666-1667 |
| DOI: | 10.1016/j.xpro.2021.101081 |
| Popis: | Summary This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) and Brill et al. (2016). Graphical abstract Highlights • Protocol for intraventricular viral injection in neonatal mice under ultrasound guidance • Neural transduction as fast as 3 dpi and expression persists until adulthood • Fast and easy technique to label and/or genetically manipulate neural cells • Same setup can be adapted to inject intramuscularly or to inject other viral vectors This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. |
| Databáze: | OpenAIRE |
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