A ‘pocket guide’ to total internal reflection fluorescence
| Autor: | Stephen E. D. Webb, Christopher J. Tynan, Marisa L. Martin-Fernandez |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Electromagnetic field
Electromagnetic theory Histology Evanescent wave Cytological Techniques 010402 general chemistry 01 natural sciences Pathology and Forensic Medicine 03 medical and health sciences Optics Microscopy Invited Reviews 030304 developmental biology Evanescent field polarization 0303 health sciences Total internal reflection Total internal reflection fluorescence microscope business.industry Chemistry Optical Imaging total internal reflection Basolateral plasma membrane Polarization (waves) 0104 chemical sciences single-molecule fluorescence imaging business TIRF microscopy |
| Zdroj: | Journal of Microscopy |
| ISSN: | 1365-2818 0022-2720 |
| Popis: | The phenomenon of total internal reflection fluorescence (TIRF) was placed in the context of optical microscopy by Daniel Axelrod over three decades ago. TIRF microscopy exploits the properties of an evanescent electromagnetic field to optically section sample regions in the close vicinity of the substrate where the field is induced. The first applications in cell biology targeted investigation of phenomena at the basolateral plasma membrane. The most notable application of TIRF is single-molecule experiments, which can provide information on fluctuation distributions and rare events, yielding novel insights on the mechanisms governing the molecular interactions that underpin many fundamental processes within the cell. This short review intends to provide a ‘one stop shop’ explanation of the electromagnetic theory behind the remarkable properties of the evanescent field, guide the reader through the principles behind building or choosing your own TIRF system and consider how the most popular applications of the method exploit the evanescent field properties. |
| Databáze: | OpenAIRE |
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